- 2010 SpringerAntonella Lanati ; in collaborazione con Luigi Cavenaghi ... [et al.] ; presentazione a cura di Marina Del Bue.
- 2006 Wileyedited by Catherine Pope, Nicholas Mays.Qualitative methods in health research / Catherine Pope, Nicholas Mays -- Qualitative interviews / Nicky Britten -- Focus groups / Jenny Kitzinger -- Observational methods / Catherine Pope, Nicholas Mays -- Conversation analysis / Sarah Collins, Nicky Britten -- Ethical issues / Dawn Goodwin -- Analysing qualitative data / Catherine Pope, Sue Ziebland, Nicholas Mays -- Quality in qualitative health research / Nicholas Mays, Catherine Pope -- Combining qualitative and quantitative methods / Alicia O'Cathain, Kate Thomas -- Case studies / Justin Keen -- Action research / Julienne Meyer -- Consensus development methods / Nick Black -- Synthesising qualitative research / Catherine Pope and Nicholas Mays.
- 2006 ScienceDirect[edited] by Constance T. Fischer.An assimilation analysis of psychotherapy : responsibility for being there / Lara Honos-Webb ... [et al.] -- Exploring psychotherapy with discourse analysis : chipping away at the mortar / Anna Madill -- The grounded theory method : application of a variant of its procedure of constant comparative analysis to psychotherapy research / David L. Rennie -- Phenomenological analysis : impression formation during a clinical assessment interview / Scott D. Churchill -- Diagnostic discourse in patient-staff interactions : a conversation analysis clarified by participant interviews / Jessie Goicoechea -- Honor and respect : feminist collaborative research with sexually abused women / Susan L. Morrow -- An empirical, phenomenological study : being joyful / Brent Robbins -- Conceptual encounter : the experience of anger / Joseph de Rivera -- Emergence of the dialogal approach : forgiving another / Steen Halling, Michael Leifer, and Jan O. Rowe -- A thematic analysis of written accounts : thinking about thought / Howard R. Pollio and Michael J. Ursiak -- Intuitive inquiry : an exploration of embodiment among contemporary female mystics / Vipassana Esbjörn-Hargens and Rosemarie Anderson -- An application of experiential method in psychology : what is it like to be a stranger in a foreign land / Arne Collen -- Focus groups and related rapid assessment methods : identifying psychoeducational HIV/AIDS interventions in Botswana / Lisa Levers.
- 2012 SpringerIvan Barofsky.The Difficulty of Assessing Quality or Quality-of-Life -- The Role of Language in Assessing Quality or Quality-of-Life -- What Role Do Cognitive Processes Play in Assessing Quality or Quality-of-Life? -- The Role of Objective or Subjective Indicators -- The Role of Signs or Symptoms -- Summary Measurement: The Role of Categories or Domains -- Symptoms and HRQOL Assessment -- Health Status and HRQOL Assessment -- Functional Status and HRQOL -- Neurocognition and HRQOL Assessment -- Well-Being as an Indicator of Quality or Quality-of-Life -- Summary and Future Directions.
- 2011edited by Stephen Gillam and A. Niroshan Siriwardena ; foreword by Iona Heath.Part 1. Background to the Quality and Outcomes Framework -- 1. Introduction: development, impact and implications / Steve Gillam and A. Niroshan Siriwardena -- 2. Where did the Quality and Outcomes Framework come from? / Martin Roland -- 3. Developing indicators and the concept of QOF ability / Stephen Campbell and Helen Lester -- Part II. Impact of the Quality and Outcomes Framework -- 4. Learning from the QOF: a review of existing research / Nicholas Steel and Sara Willems -- 5. The public health impact / Anna Dixon, Artak Khachatryan and Tammy Boyce -- 6. 'Smoke and mirrors'? Informatics opportunities and challenges / Maria Kordowicz and Mark Ashworth -- 7. The impact of the QOF on practice organisation and service delivery / Kath Checkland and Stephen Harrison -- Part III. Practical aspects of the Quality and Outcomes Framework -- 8. Getting the most out of the QOF / Chantal Simon and Anna Morton -- 9. Does the patient always benefit? / Patricia Wilkie -- Part IV. Reflections on pay-for-performance and the Quality and Outcomes Framework -- 10. Pay-for-performance schemes in primary care: what have we learnt? / Stephen Peckham and Andrew Wallace -- 11. An international perspective on the basis of pay-for-performance / Barbara Starfield and Dee Mangin -- 12. The Quality and Outcomes Framework: triumph of evidence or tragedy for personal care? / Steve Gillam and A. Niroshan Siriwardena.
- 2015 CambridgeSharon T. Mortimer, PhD, Director, Oozoa Biomedical Inc., West Vancouver, Canada, David Mortimer, PhD, Oozoa Biomedical Inc., West Vancouver, Canada.
- 2011 CRCnetBASEedited by Todd Pawlicki, Peter B. Dunscombe, Arno J. Mundt and Pierre Scalliet.
- 2011 CRCnetBASEedited by Maciej J. Bogusz.pt. 1. Quality assurance of chemometric methods and pathology : selected topics -- pt. 2. Quality assurance aspects of newly emerging methods in pathology and laboratory medicine -- pt. 3. Accreditation, standards, and education : their role in maintaining quality.
- v. 1-2, 1997-2007.v. 1. A compendium of guidelines and related materials -- v. 2. Good manufacturing practices and inspection.
- Quality assurance of pharmaceuticals : WHO guidelines, good practices, related guidance and GXP training materials.. Version 5.0.2015
- Quality assurance of pharmaceuticals : WHO guidelines, good practices, related regulatory guidance and GXP training materials.. Version 6.0.2016
- Quality assurance of pharmaceuticals : WHO guidelines, related guidance and GXP training materials, 20142014World Health Organization.
- 2013Quality assurance of pharmaceutical products is a continuing concern of WHO. Despite efforts made around the world to ensure a supply of quality and effective medicines, substandard, spurious, falsified and counterfeit products still compromise health care delivery in many countries. To respond to the global need for adequate quality assurance of pharmaceuticals, WHO's Expert Committee on Specifications for Pharmaceutical Preparations has over the years made numerous recommendations to establish standards and guidelines and to promote the effective functioning of national regulatory and control systems and the implementation of internationally agreed standards by trained personnel. More than 70 relevant documents endorsed by the Committee are reproduced in this volume, providing guidance covering all aspects of quality assurance including good manufacturing practices (GMP). This CD-ROM in addition includes a study pack with a huge set of GXP training materials.
- 2015 SpringerFeroz Jameel, Susan Hershenson, Mansoor A. Khan, Sheryl Martin-Moe, editors.1 Challenges and Opportunities for Biotech Quality by Design -- 2 Lessons Learned From Monoclonal Antibody Applications to the Office of Biotechnology Products Quality by Design Pilot Program -- 3 Definitions and Scope of Key Elements of QbD -- 4 An Overview of Quality by Design for Drug Product -- 5 Development of Drug Product Formulations: Molecular Design and Early Candidates Screening -- 6 Approaches for Early Developability Assessment of Proteins to Guide Quality by Design of Liquid Formulations -- 7 Application of QbD Principles to Late-stage Formulation Development for Biological Liquid Products -- 8 Application of QbD Principles for Lyophilized Formulation Development -- 9 Drug Substance Frozen Storage and Thawing -- 10 Quality by Design as Applied to Drug Substance Formulation using Ultrafiltration and Diafiltration -- 11 A QbD Approach in the Development and Scale-up of Mixing Processes -- 12 Application of QbD Elements in the Development and Scale-up of a Commercial Filtration Process -- 13 Application of QbD Elements in the Development and Scale-up of Commercial Filling Process -- 14 Lyophilization Process Design and Development Using QbD Principles -- 15 Visible and Subvisible Protein Particle Inspection Within a QbD-based Strategy -- 16 Quality by Design for Distribution of Environmentally Sensitive Pharmaceutical Products -- 17 Quality by Design for Primary Container Components -- 18 Devices and Combination Products for Biopharmaceuticals -- 19 Applicability of QbD for Vaccine Drug Product Development -- 20 Automation and High Throughput Technologies in Biopharmaceutical Drug Product Development with QbD Approaches -- 21 Critical Quality Attributes, Specifications, and Control Strategy -- 22 Multivariate Analysis for Process Understanding, Monitoring, Control and Optimization in Lyophilization Processes -- 23 Using Mathematical Modeling and Prior Knowledge for QbD in Freeze-drying Processes -- 24 Application of Multivariate Statistical Process Monitoring to Lyophilization Process -- 25 Application of PAT in Real-time Monitoring and Controlling of Lyophilization Process -- 26 Product Homogeneity Assessment during Validation of Biopharmaceutical Drug Product Manufacturing Processes -- 27 Application of Quality by Design Principles to the Drug Product Technology Transfer Process -- 28 Regulatory Considerations For Implementation of the QbD Paradigm for Biologics: Laying the Foundation for Product and Process Lifecycle Management.
- 2012Stefanie Duttler.One of the most critical times in the life of a protein is during its biogenesis at the ribosome. A nascent polypeptide cannot achieve its final fold while still bound to the ribosome, and is at risk of being accessed by the cellular quality control machinery. However, the extent to which cotranslational ubiquitination and degradation occur remains under debate. Conflicting reports exist, ranging from 40% of nascent chains being subject to cotranslational degradation to claims that nascent polypeptides are, on the contrary, protected from degradation. Here, we developed a direct and quantitative method to determine the extent of cotranslational ubiquitination using ribosome isolation coupled to ubiquitin-affinity purification. We find that cotranslational ubiquitination occurs at low levels, and that at least a fraction of ubiquitinated nascent chains is targeted to the proteasome for degradation. We determined which proteins are susceptible to cotranslational ubiquitination and find that intrinsic sequence features determine the cotranslational ubiquitination of a specific subset of proteins. These proteins are more aggregation-prone, highly expressed and longer than 400 amino acids. Short proteins seem to be protected from cotranslational ubiquitination. One such mechanism could be the cotranslational formation of folded structures, which lead us to disrupt cotranslational folding by deleting chaperones or through drugs such as azetidine-2-carboxylic acid (AZC). Both lead to an increase of cotranslational ubiquitination as observed by pulse-labeling as well as microarray analysis. Ribosome-associated chaperones and cotranslational folding seem to have evolved to prevent a high degree of nascent chain degradation/ubiquitination. Similarly to ribosome-associated chaperones, the ubiquitin-proteasome-system may also be able to access nascent protein chains, but rather target them for degradation. We therefore determined which parts of the UPS mediate cotranslational ubiquitination. A clear E2-E3 relationship could however not be established, possibly due to redundancy in the cellular ubiquitin ligase network. Finally, we find that disruption of mRNA quality control components also leads to enhanced ubiquitination of nascent chains, suggesting that cotranslational quality control serves to avoid the production of erroneous proteins and protect the cell from resulting toxicity.
- 2010 SpringerU. Kristoffersson, J. Schmidtke, J.-J. Cassiman, editors.Improving quality and harmonization of standards in clinical genetic services in Europe: the EuroGentest Network of Excellence -- The CanGeneTest Pan-Canadian Research Consortium on genetic laboratory services -- Regulating genetic testing: the relevance of appropriate definitions -- Genetic diseases as rare diseases: a European policy view -- European regulatory issues related to quality in provision of genetic service -- The European IVD directive and genetic testing -- Quality issues in genetics services in the United Kingdom -- The primary care perspective of quality in clinical genetics service, United Kingdom as an example -- Regulation of genetic testing/service in Canada -- Quality issues in clinical genetic services in Australia -- Clinical genetic teesting and services, the US perspective -- US oversight and regulation of genetic testing -- Regulatory issues in clinical and laboratory genetics in developing countries: examples from Latin America -- Assuring quality when establishing medical genetic services in middle- and low-income nations -- Clinical validity and utility of genetic testing in heritable disorders -- Clinical validity and utility of genetic testing in common multifactorial diseases -- The quality of genetic screening: an integral approach -- The use of prinicples in allocating scarce health care resources for genetic tests -- Outcome measures in clinical genetics services -- Direct to consumer testing -- Competency based core curriculum for training specialists in clinical genetics -- Ensuring education and quality in the practice of health professionals (non-medical) working in genetic services -- Quality issues in clinical genetic services: ethical aspects -- Democratic expert influence through bioethical advisory committees? The case of PGD legislation in Sweden -- Quality issues in clinical genetic services; regulatory issues and international conventions -- IPR issues and high quality genetic testing -- Quality issues in the evaluation and regulation of genetic testing services: a public health approach -- Quality management systems and accreditation -- External quality assessment in molecular genetic testing -- Quality issues in molecular genetic testing -- Quality issues in molecular genetic testing -- Quality in cytogenetics -- Fluorescence in situ hybridization (FISH), quality issues in molecular cytogenetics -- Quality issues in biochemical genetic testing -- Emerging technologies, need for quality assessment -- Genetic counselling in rare diseases -- Genetic counselling for late-onset disorders -- Genetic counselling for common diseases, cancer susceptibility as paradigm -- Genetic counselling in disorders of low penetrance -- Patient perspectives on genetic testing -- Index.
- 2013 SpringerFabiola Bento, Sandro Esteves, Ashok Agarwal, editors.In the last decades, major advances have been made in assisted reproductive technologies (ART) and the public demand for these procedures has increased globally. All ART clinics, from those just starting out to the well established, must employ the latest equipment and implement the best practices, while ensuring that their resources are effectively engaged to optimize patient outcomes. This is a tenet of the fiduciary role of physicians and it is increasingly recognized as a quantifiable goal regulated by formal certifications and accreditations. Quality management protocols such as those proposed by the International Organization for Standardization (ISO) are being rapidly adopted as standards of measure. Quality Management in ART Clinics: A Practical Guide provides easily adoptable ways to implement and improve formalized quality management systems. Essential to any clinic to achieve best practices and maintenance of formal regulatory certifications, this book brings together the know-how of experienced opinion leaders operating in key areas worldwide. The book offers an overview of primary regulations in the ART field, with attention to quality management demands, and links specific requirements to practical steps for implementation. Filled with process and procedure examples, flow diagrams and administrative form templates, this book is the first of its kind, gathering the necessary elements for optimizing practice, management, and quality assurance.
- 2016 Cambridgeedited by Bertrand Guidet, Professor of Medicine and Head, Medical Intensive Care Unit, Saint Antoine Hospital, Paris, France, past president, French Intensive Care society (SRLF), Andreas Valentin, Professor, Medical University of Vienna, Head of Internal Medicine, Kardinal Schwarzenberg Hospital, Schwarzach, Austria, Chair, ESCIM Working Group on Quality Improvement, founding president, Federation of Austrian Societies of Intensive Care Medicine, Hans Flaatten, Professor and Senior Consultant, Intensive Care Unit, Haukeland University Hospital, Bergen, Norway, Chair, ESICM Outcomes Section.
- H. M. Rosenberg ... [et al.].Also available: Print – 1999
- 2007Peter M. Fayers and David Machin.Introduction -- Principles of measurement scales -- Developing a questionnaire -- Scores and measurements : validity, reliability, sensitivity -- Multi-item scales -- Factor analysis and structural equation modelling -- Item response theory and differential item functioning -- Item banks, item listing and computer-adaptive tests -- Choosing and scoring questionnaires -- Clinical trials -- Sample sizes -- Cross-sectional analysis -- Exploring longitudinal data -- Modelling longitudinal data -- Missing data -- Practical and reporting issues -- Quality-adjusted survival -- Clinical interpretation -- Meta-analysis.
- Quality of life : the assessment, analysis, and reporting of patient-reported outcomes. Third edition. [3rd ed.]2016 WileyPeter M. Fayers and David Machin.Principles of measurement scales -- Developing a questionnaire -- Scores and measurement : validity, reliability, sensibility -- Multi-item scales -- Factor analysis and structural equation modelling -- Item response theory and differential item functioning -- Item banks, item linking, and computer-adaptive tests -- Choosing and scoring questionnaires -- Clinical trials -- Samples sizes -- Cross-sectional analysis -- Exploring longitudinal data -- Modelling longitudinal data -- Missing data -- Practical and reporting issues -- Death and quality-adjusted survival -- Clinical interpretation -- Biased reporting and response shift -- Meta-analysis.
- Quality of life impairment in schizophrenia, mood and anxiety disorders : new perspectives on research and treatment2007 Springeredited by Michael S. Ritsner and A. George Awad.Key methodological issues -- Distress/protection vulnerability model of quality of life impairment syndrome -- Role of dopamine in pleasure, reward and subjective responses to drugs -- Neuroendocrine functions,mood and quality of life -- In the mind of the beholder neuronal mediators for the effect of emotional experience on quality of life -- Cross-cultural quality of life research in mental health -- Measuring the value of health-related quality of life -- Comparison of instruments for measuring the quality of life impairment syndrome in severe mental disorders -- Integrative bottom-up approach to HRQQL measurement -- Quality of life impairment syndrome in severe mental disorders -- Health related quality of life in subjects at risk for a first episode of psychosis -- Quality of life impairment syndrome in schizophrenia -- Insight and quality of life in schizophrenia spectrum disorders -- Quality of life and major depression -- Quality of life impairment in bipolar disorder -- Quality of life impairment in anxiety disorders -- Quality of life in obsessive-compulsive disorder -- Treatment and rehabilitation issues -- Antipsychotic medications, schizophrenia and the issue of quality of life -- Quality of life outcomes of ECT -- Quality of life in mental health services -- Subjective quality of life in relation to psychiatric rehabilitation and daily life -- Cost-utility analysis.
- 2008 SpringerTony Fahey, Helen Russell, Christopher T. Whelan, ed.
- 2007 Springeredited by Heidrun Mollenkopf and Alan Walker.
- 2010Kendall A. Smith.Chapter 1. Introduction - The Evolution of Our Understanding of the Immune System -- Chapter 2. Molecular Immunology -- Chapter 3. The Problem - Understanding How Molecules Direct the Behavior of Cells Comprising the Immune System -- Chapter 4. The Quantal Theory of Immunity -- Chapter 5. The Variability of Cell Cycle Progression and the Competence and Progression Phases of the Cell Cycle -- Chapter 6. The Quantal Nature of IL-2-Promoted T Cell Cycle Progression -- Chapter 7. The Molecular Basis for Quantal IL-2/IL-2R Signaling of Cell Cycle Progression - The IL-2/Receptor Interaction -- Chapter 8. The Molecular Basis for Quantal IL-2/IL-2R Signaling of Cell Cycle Progression - IL-2 and IL-2 Receptor Metabolism -- Chapter 9. The Molecular Basis for Quantal IL-2/IL-2R Signaling of Cell Cycle Progression - IL-2 Receptor Signaling via the Jak/Stat Pathway -- Chapter 10. The Molecular Basis for Quantal IL-2/IL-2R Signaling of Cell Cycle Progression - IL-2 Receptor Signaling via Phosphorylation of IL-2R β Chain Y338 -- Chapter 11. The T Cell Antigen Receptor Complex and the Quantal Regulation of the IL-2 and IL-2R Genes -- Chapter 12. Digital Signaling via the T Cell Antigen Receptor Complex -- Chapter 13. Negative Feedback Regulation of T Cell Antigen Receptor Complex Signaling - Attenuation of IL-2 Gene Expression -- Chapter 14. The Paradox of the IL-2 (-/-) Mouse -- Chapter 15. The Scurfy Mouse -- Chapter 16. Lymphopenia, Autoimmunity and the Regulatory T Cell (Treg) -- Chapter 17. Treg-mediated "Active Suppression" of T Cell Proliferation -- Chapter 18. FOXP3, A Better ID-Tag for Tregs? -- Chapter 19. Mice Versus Men -- Chapter 20. Active Versus Passive Suppression and IL-2 Metabolism -- Chapter 21. FOXP3 Restricts But Does Not Suppress IL-2 Production -- Chapter 22. Both the TCR and IL-2 Regulate Foxp3 Expression -- Chapter 23. The Effects of FOXP3 Expression -- Chapter 24. Role of IL-2 in the Generation of Immune Responses In Vivo -- Chapter 25. The Role of the IL-2r Chains in IL-2 Signaling, Consumption and Suppression of T Cell Proliferation -- Chapter 26. T Cell Tissue-specific Autoimmunity -- Chapter 27. Type 1 Diabetes Mellitus (TIDM), a Prototypic Genetic Autoimmune Disease with a Tie to IL-2 -- Chapter 28. The Pathogenesis of Leukemia - Loss of Normal Quantal Growth Control.
- 2010 WHOAlso available: Print – 2010
- Quantification of hemodynamics and luminal wall motion in human abdominal aortic aneurysms using magnetic resonance imaging and computational fluid dynamics2010Andrea Seba Les.An abdominal aortic aneurysm (AAA) is a localized expansion of the abdominal aorta. AAA disease affects 5-7% of Americans over age 60 and AAA rupture kills approximately 9,000 people each year. Currently, there is no effective medical therapy for AAA disease and most AAA patients undergo "watchful waiting" until their AAA reaches the threshold for surgical repair. During the course of my doctorate studies, I used magnetic resonance imaging (MRI) and computational fluid dynamics to investigate exercise as a possible medical therapy for AAA disease and to understand the baseline flow and luminal motion conditions in the aneurysmal abdominal aorta. I imaged 64 patients with small AAAs (< 5 cm) in the supine position using a 1.5T Magnetic Resonance scanner. A 3D gadolinium-enhanced magnetic resonance angiography (MRA) sequence was used to image the aorta, a cardiac-gated phase contrast sequence (PC-MRI) was used to measure blood velocity perpendicular to the aorta at the supraceliac (SC) and infrarenal (IR) levels, and a cardiac-gated cine-FIESTA sequence was used to image luminal wall motion at the SC, IR, and Mid-Aneurysm (MA) locations. This data was used to conduct the following investigations: 1. Finite Element Analysis of blood flow at rest and simulated exercise. The MRA and PC-MRI data were used in combination with finite element analysis to elucidate the hemodynamic factors by which exercise might slow AAA growth in eight patients. Results indicate that mean wall shear stress (MWSS) was lowest in the aneurysm during rest (2.5±2.1 dynes/cm2) and MWSS increased and oscillatory shear index (OSI) decreased at the SC, IR, MA, and suprabifurcation locations during exercise. Mild turbulence existed at rest, while moderate aneurysmal turbulence was present during exercise. We hypothesize that the increased MWSS, decreased OSI, and moderate turbulence present during exercise may attenuate AAA growth. 2. Allometric scaling of mean SC and IR flow. The PC-MRI data were used to derive allometric scaling equations of mean SC and IR flows versus metrics of body size in 36 patients. Results of this investigation revealed that both the SC and IR peak-aligned averaged waveforms had the biphasic shapes characteristic of older adults. Mean SC and IR flows were 51.2±10.3 ml/s and 17.5±5.44 ml/s, respectively. Linear regression of the log-log plots of mean SC and IR flows versus body size revealed that body surface area was the strongest predictor of mean SC (R2=0.29) and IR flow (R2=0.19). 3. Wall motion characterization in the aneurysmal abdominal aorta. The cine-FIESTA luminal wall motion data were used to compute average diameter, strain, elasticity, and stiffness as well as changes in wall elastic properties versus age at the SC, IR, and MA locations in 45 patients. Results revealed that aortic elasticity and stiffness varied exponentially with age, and that the average percent change in diameter was 6.69%, 5.60%, and 2.66% at the SC, IR, and MA locations, respectively. Future work entails comparing morphologic MRI data at study intake and 2- or 3- follow-up, validating our simulated velocity fields against 2D 3-component PC-MRI data, and further developing 3D phase contrast acquisition and post-processing techniques in order to compute patient-specific aortic branch vessel flow splits, as well as for improved velocity field validation.
- 2013 CRCnetBASEedited by L. Tugan Muftuler, Department of Neurosurgery, Medical College of Wisconsin."Although traditionally a qualitative technique, MRI can be used to gather quantitative information on the structure and function of the human body. While historically applied to neurophysiology, MRI is now routinely being used to explore other organs and structures, especially the heart and skeleton. This volume reviews various MRI techniques for obtaining quantitative and physiological information of the human body. It compares and contrasts several different applications of MRI in quantitative research and on various body parts/structures, including data acquisition, processing, and analysis/interpretation. The book also explores new and emerging techniques and modeling"--Provided by publisher.
- 2011Mindy H. Chang.The visual system has a limited capacity for capturing and processing the richness and intricate detail of the surrounding environment. Visual information that arrives in the retina is converted from relative light intensities to patterns of excitation and then transmitted to a hierarchy of visual areas, which process and combine increasingly complex features of the visual signal to form a visual percept. At each stage, the amount of task or stimulus-related information a neuron can encode depends on the separability of its responses to different conditions. Using electrophysiological recordings of extracellular spiking activity from single neurons in awake behaving monkeys, we explored ways to quantify information in neuronal firing rates in order to address specific questions about sensory and cognitive signals in visual cortex and frontal cortex during different behavioral contexts. In the first study, we addressed a question of latency differences in the visual pathways that process color using a passive fixation task. Color processing occurs generally along two separate chromatic pathways, and previous work has indicated that information from the two pathways arrive with a relative lag in primary visual cortex. However, to form a perception of color, these two pathways must converge at some stage of visual processing. We used information theory to examine the timecourse of chromatic information in neurons further up the visual hierarchy in area V4, which has been implicated as an area with an important role in color processing. We found that on average, information specific to each pathway arrived simultaneously in V4, suggesting that color signals from the different pathways converge at some point within or before V4 in the visual hierarchy. In order to select behaviorally relevant information from the large amount of visual information available, shifting the focus of gaze (via saccadic eye movements) and directing attention provide ways to allocate processing resources to selected locations in visual space. Studies have shown that perceptual enhancements at behaviorally selected spatial locations are accompanied by enhanced processing in visual cortex. The mechanisms by which neurons in the brain control the selection of sensory signals remain unclear. Previous works suggest that the control of attention and eye movements, as well as the modulation of sensory representations, originate in a distributed network that includes the frontal eye field (FEF) in frontal cortex. We studied responses of single neurons recorded separately in area V4 and the FEF of monkeys engaged in different visuospatial selection tasks. In addition to firing rate responses, we examined the trial-to-trial response variability, which has been suggested to reflect behavioral state. During natural vision, the eyes make frequent movements to selected targets. To better understand how these gaze shifts influence visual processing, we examined selective visual processing in area V4 during saccade preparation. We found that V4 neurons show transiently enhanced stimulus discrimination at the saccade target. This enhancement is due in part to changes in response magnitude, but may also be facilitated by reduced variability (increased reliability) of sensory representations. The similarity to effects of covert attention and experimental manipulations of FEF activity provides further evidence that the mechanisms driving visual modulation during saccade preparation and covert spatial attention rely on common neural resources. To explore signals that likely modulate visual responses through feedback connections, we examined the role of FEF neurons in the maintenance and selection of spatial information. In a task that required remembering and directing spatial attention to a cued location while withholding eye movements, neurons in the FEF exhibited spatially selective persistent activity, which continuously tracked the location of the cue. Moreover, this maintenance of spatial information correlated with successful deployment of attention. Despite robust visual and cognitive firing rate modulations that predicted behavioral performance on the task, declines in response variability appeared to be most effectively driven by visual stimulation, rather than spatial working memory or attention. This indicates that, at least in the FEF, behavioral engagement alone is not sufficient to drive changes in variability. Instead, changes in response variability may reflect shifts in the balance between feedforward and recurrent sources of excitatory drive.
- 2006 SpringerHabib Zaidi, editor.Also available: Print – 2006
- Quantitative analysis of neural and behavioral responses to thermal gradients in the nematode Caenorhabditis elegans2007/2008Daniel Ramot.
- 2011Sun-Hae Hong.Replication and segregation of the chromosome in the bacterium Caulobacter crescentus takes place simultaneously. Although it is known that each arm of the circular chromosome is on average linearly positioned along the cell length, the detailed configuration of the DNA in the cell is not well understood. Furthermore, in replicating bacterial cells, the centromere is segregated by a ParA-dependent mechanism and anchored at the pole, but the segregation mechanism for the rest of the chromosome is not known. To address these questions, I tracked the position and motion of multiple chromosomal loci both in non-replicating and replicating cells. By characterizing compaction of the DNA in non-replicating cells, I show that the DNA in the Caulobacter cells has the mean end-to-end distance that scale as (contour length)0.22, which suggests that compaction of the bacterial DNA is primarily driven by supercoiling. Analysis of the replication/segregation dynamics revealed that Caulobacter chromosome segregation is bimodal: Centromere-proximal DNA is segregated with the centromere at a slow pace whereas the rest of the DNA is segregated much faster. The dynamics of the centromere-distal DNA are consistent with a model where continuous compaction pulls the DNA toward the pole. The results provide a new perspective on the physical configuration of the non-replicating DNA and on the movement and compaction of newly replicated DNA immediately after replication and during its transport from the replisome to the cell poles.
- Quantitative analysis of upper limb function among children with cerebral palsy during a reach and grasp cycle2011Erin Elizabeth Butler.The ability to reach, grasp, transport, and release objects is central to activities of daily living, such as feeding and grooming. However, children with cerebral palsy (CP) often have difficulty with these tasks, limiting their independence. While it is challenging to characterize and quantify specific upper limb movement disorders in CP, it is essential for identifying the underlying neural correlates and etiology, assessing movement disorder subtypes, i.e. spasticity, dystonia, and ataxia, which affect treatment selection, and measuring treatment outcomes. Current methods for measuring upper limb motion deficits are based predominantly on subjective, observational assessments. Thus, we have proposed three-dimensional motion analysis of the upper limbs during a Reach & Grasp Cycle to address the need for a standardized protocol for analysis of upper limb motion. The Reach & Grasp Cycle is a sequence of tasks that incorporates all major joints of the upper limb and simulates a functional task that is feasible yet challenging for individuals with CP. Using a biomechanical model of the trunk and upper limbs, we calculated three-dimensional joint kinematics and temporal-spatial parameters for 30 typically developing (TD) children and 25 children with CP and upper limb involvement, ages 5-18 years, using an optoelectric motion analysis system. Consistent normative data and clinically significant differences in joint motions and temporal-spatial parameters between the CP and TD children suggest the Reach & Grasp Cycle is a repeatable protocol for objective and quantitative clinical evaluation of functional upper limb motor performance. Next, we derived a single score of upper limb pathology from upper limb kinematics called the Pediatric Upper Limb Motion Index (PULMI). The root-mean-square difference was calculated between the data of each child with CP and the average from the TD population for eight kinematic variables over the Reach & Grasp Cycle. The raw value was then scaled such that a PULMI score [greater than or equal to] 100 indicated the absence of upper limb pathology, and every 10 points below 100 corresponded to one standard deviation away from the TD PULMI mean. The PULMI was significantly different between the TD children and children with CP (Wilcoxon Z=-5.06, p< .0001), and between children with spastic CP and dyskinetic CP (Z=-2.47, p< .0135). There was a strong negative correlation between the PULMI and the standard Manual Ability Classification System for all children with CP (Spearman's rho=-.78, p< .0001), indicating good validity of the PULMI. In addition, four key temporal-spatial parameters (movement time, index of curvature during reach, ratio of the peak velocity of the transport and reach phases of the Reach & Grasp Cycle, and total number of movement units) revealed differences in movement patterns between CP and TD children. Furthermore, a multi-variable logistic regression of these temporal-spatial parameters was derived which correctly predicted 19 of 22, or 86%, of movement disorder sub-types (spastic versus dyskinetic CP). This research describes a pediatric upper limb motion index (PULMI) for children with cerebral palsy (CP) that provides information regarding the quality of upper limb motion during a functional sequence of tasks. The PULMI, calculated from upper limb kinematics, and key temporal-spatial parameters of the Reach & Grasp Cycle offer a quantitative approach to analyzing the quality of upper limb function in children with CP and identifying specific types of movement deficits. It is suggested for use in both research and clinical applications.
- 2013Colin J. Fuller.Faithful propagation of the genome during division is a process fundamental to all cells. Eukaryotic cells segregate their chromosomes by attaching them via the kinetochore, a large multi-protein complex, to a microtubule spindle that physically moves the chromosomes to the daughter cells. The kinetochore is assembled at the site of the centromere, a specialized chromatin domain and set of associated proteins. In most organisms, the identity of the centromere is not determined by an underlying DNA sequence but by the incorporation of a centromere-specific histone H3 variant called CENP-A. How the cell recognizes these centromere-specific histones as the site for kinetochore assembly and the site at which to replenish CENP-A during cell division remains a major unanswered question. Many experimental techniques have been used to probe the function of the centromere, but quantitative fluorescence imaging has proved especially useful because of its molecular specificity, high sensitivity, and ability to examine single cells and single centromeres. Accurate quantitative analysis of images is critical to being able to apply fluorescence imaging to centromeres, where often meaningful intensity differences between treatments can be a factor of two or smaller, and meaningful spatial distances are far smaller than the diffraction limit of light. Empirical determination of the accuracy of an image analysis method is necessary to have confidence in these subtle measurements. We first present a series of quantitative image metaanalysis tools whose aim is to provide a quantitative metric to compare different analysis methods, and we develop an accurate and linear automated method for quantifying the intensities of centromeres in fluorescence images. Next, we describe a new superresolution microscopy methodology that can be used to measure and correct the optical aberrations introduced by cells, a critical step for measuring spatial distances at centromeres. Finally, we combine fluorescence imaging with a system for biochemical reconstitution of centromeric chromatin to examine the question of how CENP-A is recognized as the site at which the cell will incorporate additional CENP-A during cell division. We find that the six amino acids at the CENP-A C-terminus are both necessary and sufficient for this recognition.
- 2009 HighWireInternational Commission on Radiation Units and Measurements.
- 2010 CRCnetBASEYvonne Connolly Martin.Overview of quantitative drug design -- Noncovalent interactions in biological systems -- Preparation of 3D structures of molecules for 3D QSAR -- Calculating physical properties of molecules -- Biological data -- Form of equations that relate potency and physical properties -- Statistical basis of regression and partial least-squares analysis -- Strategy for the statistical evaluation of a data set of related molecules -- Detailed examples of QSAR calculations on erythromycin esters -- Case studies -- Methods to approach other structure-activity problems.
- Quantitative health risk analysis methods : modeling the human health impacts of antibiotics used in food animals2006 Springerby Louis Anthony Cox, Jr.
- 2014 ScienceDirectedited by Jennifer C. Waters, Torsten Wittmann.
- 2010Aaron Thomas Fafarman.Electrostatic fields in the interior of proteins, the consequence of the charged, polar and polarizable matter they are comprised of, have been hypothesized to vary on the order of tens of megavolts per centimeter and thus to be of tremendous consequence to biological processes. It is intuitively apparent that the rate of electron transfer in photosynthesis, the rate constant for catalysis by an enzyme, the flux through an ion channel, or the affinity between a drug molecule and its target, each involving a translocation of charged or polar species, would depend strongly on the energetic contribution from the electrostatic fields exerted by the surroundings. Despite a proliferation of calculations aimed at rationalizing the energetics of these processes, there remains a paucity of direct measurements of the electrostatic fields on which these calculations depend. By Stark spectroscopy, the directional and linear sensitivity of certain vibrational transitions to externally applied electric fields has been demonstrated, and a calibration obtained, in the form of the linear Stark tuning rate. The hypothesis has been previously submitted that for such probes, incorporated into proteins, spectroscopically observed band shifts could be quantitatively translated into changes in the electrostatic fields experienced by the probe. Carbon-fluorine and carbon-deuterium oscillators are examined as probes of electrostatic field and the means to circumvent the limitations of spectral congestion for the former and low oscillator strength for the latter are demonstrated. As an alternative solution to both problems, a straightforward and general method for the incorporation of thiocyanate electric field probes at any location in a protein by post-translational cysteine modification is presented. Incorporating nitrile probes into many locations in the proteins ribonuclease S and ketosteroid isomerase, the Stark model for vibrational band shifts is evaluated more critically than has been done previously for these probes. In ribonuclease, vibrational Stark spectra are used to calibrate multiple types of nitrile-modified proteins. The results provide evidence that the simple response to external electric fields of small, nitrile-containing molecules immobilized in frozen organic glasses can be generalized to nitriles in the interior of a protein, a requisite condition for the simple interpretation of band shifts in terms of changes in the internal electrostatic field. With this point established, the accuracy of the electrostatic force model incorporated in a molecular dynamics force field is evaluated by comparing observed spectral shifts to those calculated using simulated electrostatic fields in conjunction with the Stark model. Qualitative agreement is observed. However, the simplicity of the Stark model is complicated by the possibility of direct hydrogen-bond formation to the nitrile. This limitation is overcome using a method to both detect cases where this occurs, and to quantitatively account for this effect: a comparison of nitrile chemical shifts by NMR and frequencies by IR, each calibrated in turn by a solvatochromic model. With this additional observable, we are able to confidently ascribe spectral shifts due to mutation, pH titration and ligand binding to changes in the electrostatic fields experienced by the probes. Efforts towards employing nitrile probes to measure electric fields in the complex environment of the photosynthetic reaction center are presented.
- 2007editors, Don Hong, Yu Shyr.Statistical methodology and stochastic modeling -- Proteomics and genomics -- Survival modeling and analysis -- Mathematical models for diseases -- Computing and visualization.
- 2013Jiajing Xu.A crucial challenge for radiology is maintaining high interpretation accuracy in the face of increasing imaging workload and limited time to review and interpret the images for each patient. Variation in interpretation accuracy among radiologists is a recognized challenge. Two techniques that could help radiologists improve their interpretation are Content-based Image Retrieval (CBIR) for diagnostic support, and lesion size tracking for evaluating response to treatment. CBIR provides assists radiologists to find images from a database that are similar in terms of shared imaging features to the images they are interpreting. The performance of CBIR hinges critically on features that characterize lesions. In the first half of the talk, I will focus on the development of a novel quantitative imaging feature that describes the margin characteristics of lesions. This new margin sharpness feature is robust to variation in lesion segmentation, and achieves excellent CBIR performance in clinical datasets. Tracking lesion size in response to treatment is a crucial component for patient management as well as towards finding the best cancer therapy through clinical trials. Traditionally, tracking lesion size using serial Computed Tomography (CT) scans is largely a manual and tedious process. In the second half of the talk, I will present a novel method to automatically track and segment lymph nodes in serial CT scans. My method has achieved excellent overall segmentation performance compared to manual segmentation provided by radiologists. Ultimately, I envision that the translation of both of the above methods to the clinic will improve diagnostic accuracy, precision, and efficiency.
- 2012 Springer Protocolsedited by Katrin Marcus.Important issues in planning a proteomics experiment : statistical considerations of quantitative proteomic data / Katharina Podwojski, Christian Stephan, and Martin Eisenacher -- Whereabouts of 2D gels in quantitative proteomics / Thierry Rabilloud -- Proteome analysis with classical 2D-PAGE / Caroline May [and others] -- Fast and sensitive coomassie staining in quantitative proteomics / Nadine Dyballa and Sabine Metzger -- Silver staining of 2D dlectrophoresis gels / Thierry Rabilloud -- Differential proteome analysis using 2D-DIGE / Caroline May [and others] -- Quantitative mass spectrometry-based proteomics : an overview / Miroslav Nikolov, Carla Schmidt, and Henning Urlaub -- Robust workflow for iTRAQ-based peptide and protein quantification / Florian Beck [and others] -- Relative protein quantification by MS/MS using the tandem mass tag technology / Loic Dayon and Jean-Charles Sanchez -- Rapid approach for isobaric peptide termini labeling / Christian J. Koehler [and others] -- Isotope-coded protein label / Josef Kellermann and Friedrich Lottspeich -- Hydroponic isotope labeling of entire plants and high-performance mass spectrometry for quantitative plant proteomics / Laurence V. Bindschedler, Davinia J.S. Mills, and Rainer Cramer -- In vivo quantitative proteome profiling : planning and evaluation of SILAC experiments / Marieluise Kirchner and Matthias Selbach -- SILAC for the study of mammalian cell lines and yeast protein complexes / Heike Piechura, Silke Oeljeklaus, and Bettina Warscheid -- Post-digestion 18O exchange/labeling for quantitative shotgun proteomics of membrane proteins / Xiaoying Ye [and others] -- Application of label-free proteomics for differential analysis of lung carcinoma cell line A549 / Barbara Sitek [and others] -- Absolute quantification of proteins using standard peptides and multiple reaction monitoring / Carla Schmidt and Henning Urlaub -- Absolute multiplexed protein quantification using QconCAT technology / Philip J. Brownridge [and others] -- Practical guide to the FLEXIQuant method / Sasha Singh [and others] -- Label-free protein quantitation using weighted spectral counting / Christine Vogel and Edward M. Marcotte -- Discovering the phosphoproteome of the hydrophobic cytochrome c oxidase membrane protein complex / Stefan Helling [and others] -- KiC assay : a quantitative mass spectrometry-based approach / Yadong Huang and Jay J. Thelen -- Robust and high-throughput sample preparation for (semi- )quantitative analysis of N-glycosylation profiles from plasma samples / L. Renee Ruhaak [and others] -- quantitative redox proteomics : the NOxICAT method / Claudia Lindemann and Lars I. Leichert -- Quantitative analysis of S-nitrosylated proteins / Federico Torta and Angela Bachi -- Analysis of ubiquitinated proteome by quantitative mass spectrometry / Chan Hyun Na and Junmin Peng -- Identification of endogenous SUMO1 accepter sites by mass spectrometry / He-Hsuan Hsiao, Erik Meulmeester, and Henning Urlaub -- Search and decoy : the automatic identification of mass spectra / Martin Eisenacher [and others] -- Software tools for MS-based quantitative proteomics : a brief overview / Simone Lemeer [and others] -- iTRAQ data interpretation / Marc Vaudel [and others] -- MSQuant : a platform for stable isotope-based quantitative proteomics / Joost W. Gouw and Jeroen Krijgsveld.
- 2012 CRCnetBASEedited by Thomas E. Yankeelov, David R. Pickens, Ronald R. Price.
- 2014 ClinicalKeyedited by Julien Cohen-Adad, Claudia A.M. Wheeler-Kingshott.Rationale for Quantitative MRI of the Human Spinal Cord and Clinical Applications -- Inflammatory Demyelinating Diseases -- Traumatic Spinal Cord Injury: Acute Spinal Cord Injury and Prognosis -- Traumatic Spinal Cord Injury: Chronic Spinal Cord Injury and Recovery -- Array Coils -- Bo Inhomogeneity and Shimming -- Susceptibility Artifacts -- Ultra-High Field Spinal Cord Imaging -- Diffusion-Weighted Imaging of the Spinal Cord -- Q-Space Imaging -- Advanced Methods to Study White Matter Microstructure -- Magnetization Transfer -- T2 Relaxation -- Atrophy -- Spinal Cord fMRI -- Physiological Noise Modeling and Analysis for Spinal Cord fMRI -- Mapping the Vasculature of the Spinal Cord --Single Voxel MR Spectroscopy in the Spinal Cord: Technical Challenges and Clinical Applications -- Annex: Anatomy of the Spinal Cord.
- 2008Joseph V. Tranquillo.Neural anatomy -- Passive membranes -- Active membranes -- Propagation -- Neural branches -- Synapses -- Networks of neurons -- Extracellular recording and stimulation -- The neural code -- Applications.
- 2013 CRCnetBASEedited by Kazuhiro Imai, Sam Y.F. Li.1. Fluorogenic derivatization followed by HPLC quantification and final identification of proteins by HPLC-tandem mass spectrometry (FD-LC-MS/MS) method / Akiyo Koshiyama, Tomoko Ichibangase, and Kazuhiro Imai -- 2. Two-dimensional difference gel electrophoresis / Viola Ruddat -- 3. Mass spectrometry utilizing isotope-coded affinity tag reagents / Naoyuki Yamada -- 4. Proteomic analyses of post-translational modifications / Wei-Chi Ku and Yasushi Ishihama -- 5. Cardiovascular proteomic analysis / Toru Suzuki and Ryozo Nagai -- 6. The proteome in neurodegenerative diseases / Teruyuki Tsuji, Masahiro Aoki, and Shun Shimohama -- 7. Liver disease-related proteomics / Hirofumi Uto, Yoichiro Takami, Shuji Kanmura, and Hirohito Tsubouchi -- 8. Respiratory disease-related proteome / Masahiro Seike and Akihiko Gemma -- 9. Renal disease-related proteome / Shinya Kaname and Tadashi Yamamoto -- 10. Aging-related proteome / Tosifusa Toda -- 11. Phosphoproteomics of tumor cell lines / Hisafumi Okabe -- 12. HCV infection and mitochondria proteomics / Kyoji Moriya, Takeya Tsutsumi, Hideyuki Miyoshi, and Kazuhiko Koike -- 13. Identification of biomarkers of infectious disease using surface-enhanced laser desorption/ionisation mass spectrometry / Shea Hamilton and Paul R. Langford -- 14. Quantitative proteomic analysis of HIV infection / Li Juan Fu and Sam F.Y. Li.
- 2006 Springer Protocolsedited by Salvatore Sechi.Acrylamide: a cysteine alkylating reagent for quantitative proteomics / Illarion V. Turko and Salvatore Sechi -- Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition / Jeffrey A. Ranish, Marjorie Brand, and Ruedi Aebersold -- Stable isotope labeling by amino acids in cell culture for quantitative proteomics / Shao-En Ong and Matthias Mann -- Quantitative proteomics of mouse brain and specific protein-interaction studies using stable isotope labeling / Toshitaka Sato, Yasushi Ishihama, and Yoshiya Oda -- The absolute quantification strategy: application to phosphorylation profiling of human separase serine 1126 / Scott A. Gerber ... [et al.] -- Quantification of proteins and metabolites by mass spectrometry without isotopic labeling / Sushmita Mimi Roy and Christopher H. Becker -- The use of a quantitative cysteinyl-peptide enrichment technology for high-throughput quantitative proteomics / Tao Liu ... [et al.] -- An isotope coding strategy for proteomics involving both amine and carboxyl group labeling / Fred E. Regnier -- Proteolytic labeling with 18O for comparative proteomics studies: preparation of 18O-labeled peptides and the 18O/16O peptide mixture / Catherine Fenselau and Xudong Yao -- Tandem mass spectrometry in the detection of inborn errors of metabolism for newborn screening / František Tureček, C. Ronald Scott, and Michael H. Gelb -- Absolute quantification of specific proteins in complex mixtures using visible isotope-coded affinity tags / Yu Lu ... [et al.] -- Computational analysis of quantitative proteomics data using stable isotope labeling / Michael J. MacCoss and Christine C. Wu -- Quantitative proteomic analysis of mammalian organisms using metabolically labeled tissues / Christine C. Wu, and Michael J. MacCoss -- Quantitative proteomic analysis of phosphotyrosine-mediated cellular signaling networks / Yi Zhang, Alejandro Wolf-Yadlin, and Forest M. White.
- 2014 Springer Protocolsedited by Roberto Biassoni, Laboratorio Medicina Molecolare, Dipartimento Medicina Traslazionale, Instituto Giannina Gaslini, Genova, Italy, Alessandro Raso, Laboratorio U.O.C. Neurochirugia, Instituto Giannina Gaslini, Genova, Italy.Twenty years of qPCR: A mature technology? -- Minimum information necessary for quantitative real-time PCR experiments -- Selection of reliable reference genes for RT-qPCR analysis -- Introduction to digital PCR -- mRNA and microRNA purity and integrity: The key to success in expression profiling -- Mediator probe PCR: Detection of real-time PCR by label-free probes and a universal fluorogenic reporter -- Absolute quantification of viral DNA: The quest for perfection -- A multiplex real-time PCR-platform integrated into automated extraction method for the rapid detection and measurement of oncogenic HPV type-specific viral DNA load from cervical samples -- Real-time PCR detection of mycoplasma pneumoniae in the diagnosis of community-acquired pneumonia -- A sensible technique to detect mollicutes impurities in human cells cultured in GMP condition -- Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia -- A reliable assay for rapidly defining transplacental metastasis using quantitative PCR -- Circulating cell-free DNA in cancer -- Gene expression analysis by qPCR in clinical kidney transplantation -- Posttranscriptional regulatory networks: From expression profiling to integrative analysis of mRNA and microRNA data -- Clinical applications using digital PCR -- Developing noninvasive diagnosis for single-gene disorders: the role of digital PCR.
- 2012 Springer Protocolsedited by Scott A. Rifkin.Backcross populations and near isogenic lines / Rik Kooke, Erik Wijnker, and Joost J.B. Keurentjes -- F2 designs for QTL analysis / Yuan-Ming Zhang -- Design and construction of recombinant inbred lines / Daniel A. Pollard -- Two flavors of bulk segregant analysis in yeast / Maitreya J. Dunham -- Selecting markers and evaluating coverage / Matthew A. Cleveland and Nader Deeb -- Composite interval mapping and multiple interval mapping : procedures and guidelines for using windows QTL cartographer / Luciano Da Costa E. Silva, Shengchu Wang, and Zhao-Bang Zeng -- Design database for quantitative trait loci (QTL) data warehouse, data mining, and meta-analysis / Zhi-Liang Hu, James M. Reecy, and Xiao-Lin Wu -- Meta-analysis of QTL mapping experiments / Xiao-Lin Wu and Zhi-Liang Hu -- Using eQTLs to reconstruct gene regulatory networks / Lin S. Chen -- Estimation and interpretation of genetic effects with epistasis using the NOIA model / Jose M. Alvarez-Castro, Orjan Carlborg, and Lars Ronnegard -- Identifying QTL for multiple complex traits in experimental crosses / Samprit Banerjee and Nengjun Yi -- Functional mapping of developmental processes : theory, applications, and prospects / Kiranmoy Das [and others] -- Statistical models for genetic mapping in polyploids : challenges and opportunities / Jiahan Li [and others] -- eQTL / Lun Li, Xianghua Zhang, and Hongyu Zhao -- Genetic mapping of quantitative trait loci for disease -- related phenotypes / Marcella Devoto and Mario Falchi -- Quantitative trait locus analysis in haplodiploid hymenoptera / Jssurgen Gadau, Christof Pietsch, and Leo W. Beukeboom.
- 2013 SpringerJonathan Mamou, Michael L. Oelze, editors.Due to parallel advances in signal processing and computer hardware in the last 15 years, quantitative ultrasound techniques have reached maturity, allowing for the construction of quantitative maps or images of soft tissues. This book will focus on 5 modern research topics related to quantitative ultrasound of soft tissues: - Spectral-based methods for tissue characterization, tissue typing, cancer detection, etc.; - Envelope statistics analysis as a means of quantifying and imaging tissue properties; - Ultrasound elastography for quantifying elastic properties of tissues (several clinical ultrasound scanners now display elastography images); - Scanning acoustic microscopy for forming images of mechanical properties of soft tissues with micron resolution (desktop size scanners are now available); and - Ultrasound computer tomography for breast cancer imaging (new ultrasound tomography systems have been developed and are currently under evaluation clinically).
- 2014 Springer Protocolsedited by Adriana Fontes, Beate Saegesser Santos.
- 2010 ScienceDirectMadan Kaila and Rakhi Kaila.
- 2016 SpringerEsteban Domingo, Peter Schuster, editors.What is a quasispecies?- Historical origins and current scope -- The nucleation of semantic information in prebiotic matter -- Evolution of RNA-based networks -- Quasispecies on fitness landscapes -- Mathematical models of quasispecies theory and exact results for the dynamics -- Theoretical Models of Generalized Quasispecies -- Theories of lethal mutagenesis: from error catastrophe to lethal defection -- Estimating fitness of viral quasispecies from next-generation sequencing data -- Getting to know viral evolutionary strategies: Towards the next generation of quasispecies models -- Cooperative Interaction within RNA Virus Mutant Spectra -- Arenavirus Quasispecies And Their Biological Implications -- Models of viral population dynamics -- Fidelity variants and RNA quasispecies -- Antiviral strategies based on lethal mutagenesis and error threshold.
- 2006 SpringerE. Domingo (ed.).Also available: Print – 2006
- 2012 Wileyedited by John T. Queenan, Catherine Y. Spong, Charles J. Lockwood.Front Matter -- Colour Plates -- Factors of High-Risk Pregnancy. Overview of High-Risk Pregnancy / John T Queenan, Catherine Y Spong, Charles J Lockwood -- Maternal Nutrition / Edward R Newton -- Alcohol and Substance Abuse / William F Rayburn -- Environmental Agents and Reproductive Risk / Laura Goetzl -- Genetics. Genetic Screening for Mendelian Disorders / Deborah A Driscoll -- Screening for Congenital Heart Disease / Lynn L Simpson -- First- and Second-Trimester Screening for Fetal Aneuploidy and Neural Tube Defects / Julia Unterscheider, Fergal D Malone -- Monitoring: Biochemical and Biophysical. Sonographic Dating and Standard Fetal Biometry / Eliza Berkley, Alfred Abuhamad -- Fetal Lung Maturity / Alessandro Ghidini, Sarah H Poggi -- Antepartum Fetal Monitoring / Brian L Shaffer, Julian T Parer -- Interpreting Intrapartum Fetal Heart Tracings / Michael Nageotte -- Maternal Disease. Sickle Cell Anemia / Scott Roberts -- Anemia / Alessandro Ghidini -- Thrombocytopenia / Robert M Silver -- Inherited and Acquired Thrombophilias / Michael J Paidas -- Thromboembolic Disorders / Christian M Pettker, Charles J Lockwood -- Cardiac Disease / Stephanie R Martin, Alexandria J Hill, Michael R Foley -- Renal Disease / David C Jones -- Pregnancy in Transplant Patients / James R Scott -- Gestational Diabetes Mellitus / Deborah L Conway -- Diabetes Mellitus / George Saade -- Hypothyroidism and Hyperthyroidism / Brian Casey -- Asthma / Michael Schatz -- Epilepsy / Autumn M Klein, Page B Pennell -- Chronic Hypertension / Heather A Bankowski, Dinesh M Shah -- Systemic Lupus Erythematosus / Christina S Han, Edmund F Funai -- Perinatal Infections / Jeanne S Sheffield -- Malaria / Richard M K Adanu -- Group B Streptococcal Infection / Ronald S Gibbs -- Hepatitis / Patrick Duff -- HIV Infection / Howard L Minkoff -- Pregnancy in Women with Physical Disabilities / Caroline C Signore -- Obstetric Complications. Recurrent Spontaneous Abortion / Charles J Lockwood -- Cervical Insufficiency / John Owen -- Gestational Hypertension, Preeclampsia, and Eclampsia / Labib M Ghulmiyyah, Baha M Sibai -- Postpartum Hemorrhage / Michael A Belfort -- Emergency Care / Garrett K Lam, Michael R Foley -- Rh and Other Blood Group Alloimmunizations / Kenneth J Moise -- Multiple Gestations / Karin E Fuchs, Mary E D'Alton -- Polyhydramnios and Oligohydramnios / Ron Beloosesky, Michael G Ross -- Prevention of Preterm Birth / Paul J Meis -- Pathogenesis and Prediction of Preterm Delivery / Catalin S Buhimschi, Charles J Lockwood -- Preterm Premature Rupture of Membranes / Brian M Mercer -- Management of Preterm Labor / Vincenzo Berghella -- Placenta Previa and Related Placental Disorders / Yinka Oyelese -- Complications of Labor and Delivery. Prolonged Pregnancy / Teresa Marino, Errol R Norwitz -- Induction of Labor / Nicole M Petrossi, Deborah A Wing -- Cesarean Delivery / Michael W Varner -- Vaginal Birth after Cesarean Delivery / Mark B Landon -- Breech Delivery / Edward R Yeomans, Larry C Gilstrap -- Operative Vaginal Delivery / Edward R Yeomans -- Obstetric Analgesia and Anesthesia / Gilbert J Grant -- Patient Safety / Christian M Pettker -- Neonatal Encephalopathy and Cerebral Palsy / Maged M Costantine, Mary E D'Alton, Gary D V Hankins -- Procedures. Genetic Amniocentesis and Chorionic Villus Sampling / Ronald J Wapner -- Fetal Surgery / Robert H Ball, Hanmin Lee -- Index.
- 2006 ScienceDirectJim Melton and Stephen Buxton.XML -- Querying -- Querying XML -- Metadata, an overview -- Structural metadata -- The XML information set (Infoset) and beyond -- Managing XML : transforming and connecting -- Storing : XML and databases -- XPath 1.0 and XPath 2.0 -- Introduction to XQuery 1.0 -- XQuery 1.0 definition -- What's missing? -- XQuery APIs -- SQL/XML -- XML-derived markup languages -- Internationalization : putting the "W" in "WWW" -- Finding stuff.
- 2013Georges C. Benjamin, Theodore Brown, Susan Ladwig, Elyse Berkman.
- 2014 ScienceDirectGeorge R. Blumenschein.This original fourteen chapter book is a brief, slightly autobiographic tale of medical oncologists, surgeons, radiation oncologists, and breast cancer patients in a well-established cancer center in Texas, who pursued the goal of cure for breast cancer. The evolution of improved outcomes in the treatment of microscopic metastatic breast cancer is also the story of the development of adjuvant chemotherapy for post-operative breast disease. The adjuvant therapy of breast cancer came about with the realization that this malignancy, when diagnosed in most patients, had spread beyond the confines of the primary cancer.
- 2012edited by Brian D. Hodges and Lorelei Lingard ; with a foreword by M. Brownell Anderson.Introduction / Brian D. Hodges and Lorelei Lingard -- 1. The shifting discourses of competence / Brian D. Hodges -- 2. Rethinking competence in the context of teamwork / Lorelei Lingard -- 3. Perturbations: the central role of emotional competence in medical training / Nancy McNaughton and Vicki LeBlanc -- 4. Competence as expertise: exploring constructions of knowledge in expert practice / Maria Mylopoulos -- 5. Assessing competence: extending the approaches to reliability / Lambert W.T. Schuwirth and Cees P.M. van der Vleuten -- 6. Blinded by "insight": self-assessment and its role in performance improvement / Kevin W. Eva, Glenn Regehr, and Larry D. Gruppen -- 7. The competent mind: beyond cognition / Annie S.O. Leung, Ronald M. Epstein, and Carol-Anne E. Moulton.
- Questioning the premedical paradigm : enhancing diversity in the medical profession a century after the Flexner report2010 ProQuest Ebook CentralDonald A. Barr."One hundred years ago, Abraham Flexner's report on Medical Education in the United States and Canada helped establish the modern paradigm of premedical and medical education. Barr's research finds the system of premedical education that evolved to be a poor predictor of subsequent clinical competency and professional excellence, while simultaneously discouraging many students from under represented minority groups or economically disadvantaged backgrounds from pursuing a career as a physician. Analyzing more than fifty years of research, Barr shows that many of the best prospects are not being admitted to medical schools with long-term adverse consequences for the U.S. medical profession."--BOOK JACKET.Also available: Print – 2010
- Cynthia Ho.
- Questions and answers on the essentials of physiology : prepared especially for students of medicine.. [1st]- ed.Google BooksHare, H. A.; Raymond, Joseph H.; Hare, H. A.Also available: Print – 1888.
- 2008 SpringerRyoichi Oyasu, Ximing J. Yang, Osamu Yoshida.
- 2010 AccessSurgeryedited by Gerard M. Doherty.A diagnostic index of over 100 symptoms and signs.
- 2016 SpringerJeanne Duus Johansen, Jean-Pierre Lepoittevin, Jacob P. Thyssen, editors.Immunology: A Quick Guide -- Clinical Features of Contact Dermatitis -- Patch Testing: Essentials -- Patch Testing with Own Products -- Allergic Contact Dermatitis: Clinical Relevance and Exposure Assessment of Allergens -- Irritant Contact Dermatitis: Diagnosis and Risk Factors -- Photoreactions and Testing -- Protein Contact Dermatitis and Testing -- Occupational Contact Dermatitis -- Contact Allergy in Children -- Main Allergen Groups: Metals -- Fragrances -- Preservatives. Allergens in Various Products: Textiles and Hair Dyes -- Dental Materials -- Shoes/Gloves -- Glues -- Metalworking Fluids -- Plants -- Cosmetics -- Workers Protection: Gloves, Creams -- Overview of Allergens.
- 2012 SpringerMazen M. Sinjab.
- 2013 SpringerBrandon Allen, Latha Ganti, Bobby Desai.For important information you need to know, but can't remember, turn to QUICK HITS IN EMERGENCY MEDICINE. This practical, short compendium contains the key decision-rules, clinical values, mnemonics, and dosages that you need at your finger tips in the ED. It covers everything from acute coronary syndrome and ACLS, to electrolyte equations and hyperkalemia, to ventilator settings by disease to Wellen's syndrome. Information on each topic is limited to a single page and presented in easily digested formats such as tables, algorithms, bulleted lists, and clinical illustrations.
- 2016 AccessMedicineMaxine A. Papadakis, Stephen J. McPhee.
- 2014 AccessMedicineMcPhee, Stephen J.; Papadakis, Maxine A.; Rabow, Michael W."Provides practical, expert information on diagnosis and management when you just have a few minutes. It is a single-source reference designed for quick and easy access to the information you need in the clinical setting"--Pref.
- 2011 SpringerNatasha Rekhtman, Justin A. Bishop.
- 2016 OvidNancy H. Diepenbrock, RN, CCRN, Eagle River, Wisconsin.Neurologic system -- Cardiovascular system -- Pulmonary system -- Gastrointestinal and urinary systems -- Renal system -- Endocrine system -- Hematologic and immune systems -- Drugs, doses, tables -- Labs -- Imaging -- Miscellaneous.
- pt. A-B, 2004. ScienceDirectpt. B ScienceDirectedited by Helmut Sies, Lester Packer.Also available: Print – pt. A-B, 2004.
- 2009 Springeredited by S. Elm, Stefan N. Willich.Medical healing implies knowledge of the assumptions that underlie our understanding of 'health', and, concomitantly, how we define well being and its opposites, illness and disease. Today, health, health care (business, wellness, recreation), and medicine (especially research-driven scientific medicine) have become separate entities with different institutions, budgets, marketing philosophies and 'corporate cultures'. Furthermore, healing is individual and subjective, yet at the same time also culturally determined. The present volume brings together papers on these topics in an unique interdisciplinary approach. The book provides an ethical framework for healthcare from a political perspective. It discusses definitions of the terminology of healing and health and their ethical and medical implications including their historical contexts. A separate section expands the theme of the cultural constructedness of healing by the concepts of traditional Chinese medicine and homeopathy. Modern medicine has a strong focus on acute care, which urgently needs to place greater emphasis on preventive medicine including the crucial importance of social factors on health and on the emergence of 'public health'. The point of view of Business Concepts, their potential and limitations are by no means neglected and the legal ramifications of genetic research and innovative medical strategies with regard to some of our most foundational notions are discussed.
- 2011 Springer Protocolsedited by Kendra P. Rumbaugh.Bioassays of quorum sensing compounds using Agrobacterium tumefaciens and Chromobacterium violaceum -- Detection of 2-alkyl-4-quinolones using biosensors -- FRET-based biosensors for the detection and quantification of AI-2 class of quorum sensing compounds -- Isolation of agr quorum sensing autoinducers -- Liquid chromatography/mass spectrometry for the detection and quantification of N-acyl-l-homoserine lactones and 4-hydroxy-2-alkylquinolines -- Detection of autoinducer (AI-2)-like activity in food samples -- Detection of bacterial signaling molecules in liquid or gaseous environments -- Determination of acyl homoserine lactone and tetramic acid concentrations in biological samples -- Luminescent reporters and their applications for the characterization of signals and signal-mimics that alter LasR-mediated quorum sensing -- Modulation of mammalian cell processes by bacterial quorum sensing molecules -- Imaging N-acyl homoserine lactone quorum sensing in vivo -- Defining the structure and function of acyl-homoserine lactone autoinducers -- Global expression analysis of quorum-sensing controlled genes -- Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system -- Quantifying pseudomonas aeruginosa quinolones and examining their interactions with lipids -- Linking quorum sensing regulation and biofilm formation by Candida albicans -- Design of synthetic mammalian quorum-sensing systems -- Qualitative and quantitative determination of quorum sensing inhibition in vitro -- Custom synthesis of autoinducers and their analogues -- Heterologous overexpression, purification, and in vitro characterization of ahl lactonases -- High-performance liquid chromatography analysis of N-acyl homoserine lactone hydrolysis by paraoxonases -- Generation of quorum quenching antibodies.
- Harrison's Principles of Internal Medicine
- AAP Red Book Online
- Robbins & Cotran Pathologic Basis of Disease
- Sabiston Textbook of Surgery
- Nelson's Textbook of Pediatrics
- Surgical Exposures in Orthopaedics
- Mandell, Douglas, & Bennett's Principles & Practice of Infectious Diseases
- Red Book Online
- ICU Book
- Primary Care Medicine
- Campbell-Walsh Urology
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Cochrane reviews are created through a strict process of compiling and analyzing data from multiple randomized control trials to ensure comprehensiveness and reliability.Provides systematic coverage of the psychological literature from the 1800s to the present through articles, book chapters and dissertations.BMJ Clinical Evidence. A clinical information tool built around systematic reviews summarizing the current state of knowledge about prevention and treatment of clinical conditions.PIER (Physicians' Information and Education Resource) is a Web-based decision-support tool designed for rapid point-of-care delivery of up-to-date, evidence-based guidance for primary care physicians.Cochrane Central Register of Controlled Trials (CENTRAL) provides access to 300,000 controlled trials that have been identified the Cochrane Collaboration.Provides drug information targeted for patients.A continually updating drug monograph.The National Guideline Clearinghouse (NGC): A comprehensive database of evidence-based clinical practice guidelines and related documents.MedlinePlus: A repository of health information from the National Library of Medicine. Links are from trusted sites. No advertising, no endorsement of commercial companies or productsLPCH CareNotes via MicroMedex: Patient education handouts customized by LPCH clinical staffMicromedex Lab Advisor: Evidence based laboratory test informationA drug database organized by generic name, trade name and drug class.LPCH / Stanford Hospital Formulary.A goldmine of trusted consumer health information from the world's largest medical library.A trusted source of expert advice for and about kids, providing the information necessary to help patients and parents understand their unique needs.Provides patient handouts from the American Academy of Family Physician.Access to the Stanford Health Library for patients.Lane provides access to over 5,000 eBooks many of which provide helpful background material that will prepare you to better tackle primary literature.
Largest, broadest eBook package; covers all sciences, as well as technology (including software), medicine, and humanities.
In addition to covering Wiley and Springer, MyiLibrary is also the only provider for Oxford and Cambridge University Press titles. No seat restrictions.A collection of biomedical books that can be searched directly by concept, and linked to terms in PubMed abstracts.
A web-based, decision support system for infectious diseases, epidemiology, microbiology and antimicrobial chemotherapy. The database, updated weekly, currently includes 337 diseases, 224 countries, 1,147 microbial taxa and 306 antibacterial (-fungal, -parasitic, -viral) agents and vaccines.
Over 10,000 notes outline the status of specific infections within each country.
Provides online, full-text access to Springer's journal titles as well as journals from other publishers.
Subjects include: life sciences, chemical sciences, environmental sciences, geosciences, computer science, mathematics, medicine, physics and astronomy, engineering and economics. Also includes eBooks.Collection of over 8 thousand fulltext titles in engineering, math, and basic and applied biomedical research. Coverage is from 1967 to the present.A library of ebooks on a wide array of topics, digitized and made available online in conjunction with the original publishers.