ArticleVan der Pijl RJ, Ma W, Lewis CTA, Haar L, Buhl A, Farman GP, Rhodehamel M, Jani VP, Nelson OL, Zhang C, Granzier H, Ochala J.
Mol Metab. 2024 Dec 16;92:102084.
AIM: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals.
METHODS: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics.
MAIN FINDINGS: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability.
CONCLUSIONS: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.