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- ArticleZviagintseva IuV, Mamedov ZM, Sal'kova EG.Prikl Biokhim Mikrobiol. 1978 May-Jun;14(3):446-9.Two isoforms of the malic enzyme from Renet Simirenko apple pulp were detected by polyacrylamide gel electoresis. The malic enzyme protein constituted the major portion of the protein extracted from apples.