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- ArticleBorderon E, Horodniceanu T, Buissiere J, Barthez JP.Ann Microbiol (Paris). 1977 May-Jun;128A(4):413-7.A dwarf colony mutant of Escherichia coli was isolated from the urine of a patient with an asymptomatic urinary infection. This dwarf mutant growths poorly (minute transparent colonies) on Trypticase Soja and Mueller-Hinton agar, and requires thiamine concentration of 3 x 10-11 M.