ArticlePeltier AP, Aussel C, Haim T, Cyna L.
J Immunol Methods. 1977;16(2):153-63.
The soluble ribonucleoprotein nuclear antigen reactive with specific antibodies present in sera from patients with Mixed Connective Tissure disease has alpha 2-beta 1 electrophoretic mobility, thus enabling its reaction with specific serum antibodies to be studied by counter-immunoelectrophoresis. Its molecular weight determined by elution from a calibrated Sephadex G-200 gel column is about 175,000. Its purification has been attempted by successive DEAE cellulose chromatography, Sephadex G-200 gel filtration and Hydroxyapatite chromatography. Polyacrylamide gel electrophoresis of RNP antigen-containing fractions showed persistent heterogeneity which could be due either to inadequate purification or to dissociation of the RNP-antigen during electrophoresis.