BookPranab Dey.
Summary: The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.
Contents:
Chapter 1: Fixation of histology samples: Principles, methods and types of fixatives
Chapter 2: Processing of tissue in the histopathology laboratory
Chapter 3: Embedding of tissue in histopathology
Chapter 4: Decalcification of bony and hard tissue for histopathology processing
Chapter 5: Tissue Microtomy: principle and procedure
Chapter 6: Frozen section: Principle and procedure
Chapter 7: Staining principle and general procedure of staining of the tissue
Chapter 8: Haematoxylin and Eosin stain of the tissue section
Chapter 9: Special Stains for the carbohydrate, protein, lipid, nucleic acid and pigments
Chapter 10: Connective tissue stain: Principle and procedure
Chapter 11: Amyloid staining
Chapter 12: Stains for the Microbial Organisms
Chapter 13: Cytology sample procurement, fixation and processing
Chapter 14: Routine staining in cytology laboratory
Chapter 15: The basic technique of fine needle aspiration cytology
Chapter 16: Immunocytochemistry in histology and cytology
Chapter 17: Flow cytometry: Basic principles, procedure and applications in pathology
Chapter 18: Digital pathology
Chapter 19: Automation in the laboratory and Liquid-based cytology
Chapter 20: Polymerase chain reaction: Principle, technique and applications in pathology
Chapter 21: Fluorescent in situ hybridisation techniques in pathology: Principle, technique and applications
Chapter 22: Tissue microarray in pathology: Principal, technique and applications
Chapter 23: Sanger sequencing and next-generation gene sequencing: Basic principles and applications in pathology
Chapter 24: Liquid biopsy: Basic principles, techniques and applications
Chapter 25: Artificial neural network in Pathology: Basic principles and applications
Chapter 26: Compound Light microscope and other different microscopes
Chapter 27: Fluorescence microscope, confocal microscope and other advanced microscopes: Basic principles and applications in pathology
Chapter 28: Electron microscopy: Principle, components, optics and specimen processing
Chapter 29: Quality control and laboratory organization
Chapter 30: Laboratory safety and laboratory waste disposal
Multiple choice questions for the self-assessment.