BookAna Valéria Colnaghi Simionato, editor.
Summary: This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.
Contents:
The new omics era into systems approaches: what is the importance of separation techniques?
Biological Applications for LC-MS-Based Proteomics
The role of chromatographic and electromigration techniques in Foodomics
CE-MS for proteomics and intact protein analysis
Peptidomics and Capillary Electrophoresis
Discovery of native protein complexes by liquid chromatography followed by quantitative mass spectrometry
Capillary electrophoresis based N-Glycosylation analysis in the biomedical and biopharmaceutical fields
Practical considerations in method development for gas chromatography-based metabolomic profiling
Capillary electrophoresis-mass spectrometry for metabolomics: possibilities and perspectives
Liquid Chromatography - Mass Spectrometry for Clinical Metabolomics: An Overview
Analytical platforms for mass spectrometry-based metabolomics of polar and ionizable metabolites
Metabolomic data treatment: basic directions of the full process
Index.