Bookedited by Bin Tian.
Contents:
Application and design considerations for 3'-end sequencing using click-chemistry / Madeline K. Jensen, Nathan D. Elrod, Hari Krishna Yalamanchili, Ping Ji, Ai Lin, Zhandong Liu, and Eric J. Wagner
PAS-seq 2 : A fast and sensitive method for global profiling of polyadenylated RNAs / Yoseop Yoon, Lindsey V. Soles, and Yongsheng Shi
TRENDseq : A highly multiplexed high throughput RNA 3' end sequencing for mapping alternative polyadenylation / Anton Ogorodnikov and Sven Danckwardt
QPAT-seq, a rapid and deduplicatable method for quantification of poly(A) site usages / Juncheng Lin, Congting Ye, and Qingshun Q. Li
Using TIF-Seq2 to investigate association between 5' and 3' mRNA ends / Bingnan Li, Sueli Marques, Jingwen Wang, and Vicent Pelechano
Single-molecule polyadenylated tail sequencing (SM-PAT-Seq) to measure polyA tail lengths transcriptome-wide / Steven L. Coon, Tianwei Li, James R. Iben, Sandy Mattijssen, and Richard J. Maraia
3' end sequencing of pA⁺ and pA⁻ RNAs / Guifen Wu, Manfred Schmid, and Torben Heick Jensen
Comprehensive profiling of mRNA polyadenylation in specific cell types in vivo by cTag-PAPERCLIP / R. Samuel Herron and Hun-Way Hwang
A computational pipeline to infer alternative poly-adenylation from 3' sequencing data / Hari Krishna Yalamanchili, Nathan D. Elrod, Madeline K. Jensen, Ping Ji, Ai Lin, Eric J. Wagner, and Zhandong Liu
Systematic refinement of gene annotations by parsing mRNA 3' end sequencing datasets / Pooja Bhat, Thomas R. Burkard, Veronika A. Herzog, Andrea Pauli, and Stefan L. Ameres
Computational analysis of alternative polyadenylation from standard RNA-seq and single-cell RNA-seq data / Yipeng Gao and Wei Li
Quantifying alternative polyadenylation in RNAseq data with LABRAT / Austin E. Gillen, Raeann Goering, and J. Matthew Taliaferro
Poly(A) tail dynamics : Measuring polyadenylation, deadenylation and poly(A) tail length / Michael Robert Murphy, Ahmet Doymaz, and Frida Esther Kleiman
Reconstitution and biochemical assays of an active human histone pre-mRNA 3'-end processing machinery / Yadong Sun, Wei Shen Aik, Xiao-Cui Yang, William F. Marzluff, Zbigniew Dominski, and Liang Tong
Comprehensive RNP profiling in cells identifies U1 snRNP complexes with cleavage and polyadenylation factors active in telescripting / Zhiqiang Cai, Byung Ran So, and Gideon Dreyfuss
Simultaneous studies of gene expression and alternative polyadenylation in primary human immune cells / Joana Wilton, Michael Tellier, Takayuki Nojima, Angela M. Costa, Maria Jose Oliveira, and Alexandra Moreira
RIPiT-Seq : A tandem immunoprecipitation approach to reveal global binding landscape of multisubunit ribonucleoproteins / Zhongxia Yi and Guramrit Singh
Generation of 3'TR knockout cell lines by CRISPR/Cas9-mediated genome editing / Sibylle Mitschka, Mervin M. Fansler, and Christine Mayr
Modulation of alternative cleavage and polyadenylation events by dCas9-mediated CRISPRpas / Jihae Shin, Ruijia Wang, and Bin Tian.