Bookedited by Daniel L. Minor Jr., Henry M. Colecraft.
Contents:
Effective production of oligomeric membrane proteins by EarlyBac-insect cell system / Hiro Furukawa, Noriko Simorowski, and Kevin Michalski
Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies / Lejla Zubcevic
Production and purification of TRPV2 and TRPV5 for structural and functional studies / Edwin C. Fluck, Ruth A. Pumroy, and Vera Moiseekova-Bell
Sample preparation of the human TRPA1 ion channel for cryo-EM studies / Yang Suo and Seok-Yong Lee
Expression and purification of the cardiac sodium channel Nav1.5 for cryo-EM structure determination / Daohua Jiang, Tamer Gamal El-Din, Ning Zheng, and William A. Catterall
Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy / Huaizong Shen, Nieng Yan, and Xiaojing Pan
Production and purification of ATP-sensitive potassium channel particles for cryo-electron microscopy / Camden M. Driggers and Show-Ling Shyng
Production of K2P2.1 (TREK-1) for structural studies / Haerim Lee, Marco Lolicato, Cristina Arrigoni, and Daniel L. Minor Jr.
Purification of a native nicotinic receptor / Md. Mahfuzur Rahman, Brady T. Worrell, Michael H.B. Stowell, and Ryan E. Hibbs
SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs / David M. Kern and Stephen G. Brohawn
Studying ion channel conformation dynamics by encoding coumarin as unnatural amino acid / Sebastian E. Brauchi and Ximena P. Steinberg
VoltageFluor dyes and fluorescence lifetime imaging for optical measurement of membrane potential / Anneliese M.M. Gest, Susanna K. Yaeger-Weiss, Julia R. Lazzari-Dean, and Evan W. Miller
Fluorescent toxins as ion channel activity sensors / Robert Stewart, Bruce E. Cohen, and Jon T. Sack
Probing ion channel macromolecular interactions using fluorescence resonance energy transfer / Sharen Rivas, Khadija Hanif, Nourdine Chakouri, and Manu Ben-Johny
Manipulation of ion channel gating with photoswitchable tweezers / Kate Dunning and Thomas Grutter - Determining stoichiometry of ion channel complexes using single subunit counting / Rikard Blunck.