BookPranab Dey.
Summary: The book covers the essential practical techniques of flow cytometry in detail. It is divided into two sections: The first section includes the basic practical techniques of flow cytometry in cytology samples. Chapters under this section provide detailed description of the sampling technique, processing, acquisition of the sample, instrumentation and basic principles of flow cytometry. The second section elucidates clinical applications of flow cytometry. Chapters cover the flow cytometry applications in various haematolymphoid neoplasms, tumors of solid organs and body fluid samples. The flow cytometry findings of different tumors are described with the help of multiple colored cytology microphotographs, flow cytometry graphs, boxes, and tables. In addition, it also describes other ancillary techniques in those neoplastic lesions. The book helps practicing pathologists, technical staff and post graduate students to understand flow cytometry findings of the haematolymphoid neoplasms and solid tumor with special emphasis on cytology along with advanced technique. This book will help the students to interpret flow cytometry graphs.
Contents:
Part I: Technical aspects
Introduction and history of flow cytometry
Basic principles of flow cytometry and instrumentation
Specimen preparation and data acquisition
Interpretation of graphs and quality control
Fluorescent probes and different useful markers for flow cytometry
Nuclei acid dye for flow cytometry
Part II: Applications of flow cytometry
Application of flow cytometry in lymphoid neoplasms
Application of flow cytometry in body cavity fluids
Application of flow cytometry in solid tumors
Cell cycle analysis, DNA flow cytometry and ploidy analysis
Future applications of flow cytometry
Practical cases in flow cytometry.