BookHiromu Yawo, Hideki Kandori, Amane Koizumi, Ryoichiro Kageyama, editors.
Summary: This book, now in a thoroughly revised second edition, offers a comprehensive review of the rapidly growing field of optogenetics, in which light-sensing proteins are genetically engineered into cells in order to acquire information on cellular physiology in optical form or to enable control of specific network in the brain upon activation by light. Light-sensing proteins of various living organisms are now available to be exogenously expressed in neurons and other target cells both in vivo and in vitro. Cellular functions can thus be manipulated or probed by light. The new edition documents fully the extensive progress since publication of the first edition to provide an up-to-date overview of the physical, chemical, and biological properties of light-sensing proteins and their application in biological systems, particularly in neuroscience but also in medicine and the optical sciences. Underlying principles are explained and detailed information provided on a wide range of optogenetic tools for the observation and control of cellular signaling and physiology, gene targeting technologies, and optical methods for biological applications. In presenting the current status of optogenetics and emerging directions, this milestone publication will be a "must read" for all involved in research in any way related to optogenetics.
Contents:
Part 1. Light-sensitive channels and transporters
1. History and Perspectives of Light-Sensing Proteins
2. Phototactic behavior of Chlamydomonas
3. Structure-Functional Analysis of rhodopsin light sensors
4. Molecular Dynamics of Channelrhodopsin
5. Photochemistry of Halorhodopsin
6. Spectroscopic Dynamics of Microbial Rhodopsins
7. Molecular Engineering of Retinylidene Proteins
8. A new optogenetic tools from deep ocean
Part 2. Light-sensitive signaling molecules
9. Biology of Light-Sensing Proteins in Plants and Microorganisms
10. Optogenetic Potentials of Diverse Animal Opsins
11. Enzyme rhodopsins
12.Optogenetic photoswitches
13.Genetic, biochemical and biophysical studies on flavoprotein photoreceptors
Part 3. Optogenetics in Biological System
14. General Description: Future Prospects of Optogenetics
15. Optogenetic Manipulation and Probing
16. Visualization and Manipulation Signaling Molecules In Vivo
17. Optical Manipulation of Gene Expression
18. Optical Manipulation of Intracellular Signaling
19. Single Molecule Optogenetics
20. Bioluminescence Imaging
21. Luminescence Optogenetics
22. Optogenetic Imaging of Protein Activity in the Synapse Using 2-Photon Fluorescence Lifetime Imaging Microscopy
23. Optogenetics in Drosophila
24. Optogenetics in Caenorhabditis elegans
25. Optogenetics in zebrafish
26. Optogenetics Research Using the Mouse as a Model System
27. Optogenetics Research Using Rat
28. Optogenetics Research Using Primates
29. Organelle Optogenetics
Part 4. Optogenetics in Neuroscience
30. Neuroscientific Frontline of Optogenetics
31. Elucidation of Neuronal Circuitry Involved in the Regulation of Sleep/Wakefulness Using Optogenetics
32. Multiscale neural activity in the behaving brain
33. Optogenetic Analysis of Striatal Connections to Determine Functional Connectomes
34. Optogenetics for the Behavior Manipulation of Non-human Primates
35. Activity Regulation in the Study of Neural Plasticity
36. Optogenetic Control of Synaptic Plasticity
37. Casting Light on the Role of Glial Cells in Brain Function
38. Optogenetic manipulation of Respiratory Rhythm
39. Optogenetic mapping of brain circuitry
40. In vivo imaging of Arc transcription
41. Neural Circuits for the Formation and Retrieval of Episodic Memories
Part 5. Medical Optogenetics
42. Medical Optogenetics, Overview
43. Towards Understanding the Neural Mechanism of Behavioral Phenotypes Seen in Psychiatric Disorders
44. Gene Therapy Using Channelrhodopsins to Treat Blindness
45. Optogenetic Approaches to Restoring Intrinsic Visual Processing Features in Retinal Ganglion Cells
46. Application of optogenetics to epilepsy research
Part 6. Opto-electro-nano Technologies for Optogenetics
47. CMOS-Based Neural Interface Device for Optogenetics
48. OLED System for Optogenetics
49. Micro-imaging probes, Opto-electrodes, and Virtual Reality Systems for Optogenetics
50. Upconversion
51. Optical probes for in vivo optogenetics.