Bookedited by Maarten Merkx.
Contents:
Preface / Maarten Merkx
1.Understanding and applications of Ser/Gly linkers in protein engineering / Eric Ceballos-Alcantarilla and Maarten Merkx
2.Design and construction of chimeric linker linkers with controllable flexibilities for precision protein engineering / Ziliang Huang, Chong Zhang and Xin-Hui Xing
3.Engineering crowding sensitivity into protein linkers / Theodoros Pittas, Weiyan Zuo and Arnold J. Boersma
4.protaTETHER: A method for the incorporation of linkers in biomacromolecules / Anna I. Wurz, Collin T. O'Bryant and Robert M. Hughes
5.Optimizing the linker length for fusing an alcohol dehydrogenase with a cyclohexanone monooxygenase Alejandro Gran-Scheuch, Friso Aalbers, Yannick Woudstra, Loreto Parra and Marco W. Fraaije
6.Predicting the effect of disordered linkers on effective concentrations and avidity with the Ceff calculator app / Magnus Kjaergaard, Juliana Glavina and Lucia Beatriz Chemes
7.ER/K-link—Leveraging a native protein linker to probe dynamic cellular interactions / Tejas M. Gupte, Michael Ritt and Sivaraj Sivaramakrishnan
8.Design of helical linkers for fusion proteins and protein-based nanostructures / Ryoichi Arai
9.Synthetic protein switches: Combinatorial linker engineering with iFLink / C Alexander Gräwe, Jan Ranglack, Anastasia Weyrich and Viktor Stein
10.Enzyme-DNA chimeras: Construction, allostery, applications / Chiao-Yu Tseng, Yong Wang and Giovanni Zocchi
11.The Spy that links: Creation of nonlinear protein architectures and materials using SpyTag/SpyCatcher chemistry / Ri Wang and Fei Sun.