BookDan S. Tawfik.
Contents:
1. Engineering lytic polysaccharide monooxygenases (LPMOs) / Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink
2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases / Daniela M. Carminati, Eric J. Moore and Rudi Fasan
3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries / Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer
4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution / Duncan S. Macdonald and Donald Hilvert
5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling / Lukas Deweid, Adem Hadjabdelhafid-Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier
6. Engineering aldolases for asymmetric synthesis / Mikael Widersten
7. Ultra-high throughput screening for novel protease specificities / Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke
8. Directed evolution of transcriptional switches using dual-selector systems / Yuki Kimura and Daisuke Umeno
9. Methods for competitive enrichment and evaluation of superior DNA ligases / Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley
10. Directed evolution of custom polymerases using droplet microfluidics / Derek Vallejo, Ali Nikoomanzar and John C. Chaput
11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution / Pu Xue, Tong Si and Huimin Zhao
12. Split enzymes: Design principles and strategy / Shion A. Lim and James A. Wells.