BookNikolai Vekshin.
Summary: In this monograph, the author--Head researcher at the Institute of Cell Biophysics of Russian Academy of Sciences--discusses the results of his own long-term studies of mitochondria as well as alternative points of view and experiments of other important researchers in the field. The monograph contains the main aspects of mitochondrial research by a number of physical methods: fluorescence spectroscopy, UV-vis spectrophotometry, IR spectroscopy, light-scattering, optical microscopy, fluorescence microscopy, colorimetry, photobleaching, polarography, among others. The monograph is very useful for researchers and graduate students specializing in mitochondrial biophysics, biochemistry, molecular biology and cytology. The book was published in Russian in 2019 by Photon Publishers (Pushchino, Moscow region).
Contents:
The mitochondria in the cell
Early studies
Structural features
Are there giant mitochondria and reticulum
Evolutionary origins
Reproduction of mitochondria in cell
Mitochondrial DNA
Membrane and matrix proteins
Bioenergetics
The respiratory chain
NADH-dehydrogenase
Succinate dehydrogenase
Cytochrome oxidase
Interactions in enzyme-substrate complexes
Mechanisms of electron transfer
Membrane synthesis of ATP
Proton transport and the chemiosmotic hypothesis
On the measurement of transmembrane potential
The conformational hypothesis
Thermal coupling model
Photo-respiration and photo-induced synthesis of ATP
Surface photodesorption
Flavin and ubiquinone of NADH-dehydrogenases are not involved in the transfer of electrons to artificial acceptors
The lag-period and the pseudo-oscillations in redox-reactions of NADH with DHPIP
Electron-conformational properties of the flavoprotein fragment of NADH-dehydrogenase
Resonant energy transfer from mitochondrial proteins to NADH
Flavin loss from NADH-dehydrogenase complex
Stabilization of NADH-dehydrogenase by adenosine phosphates
Dehydrogenase mitochondrial activity in the touch, determined by the decrease in tryptophan fluorescence by formosan
Rotenone-insensitive NADH oxidation by respiratory chain fragments
Cytochrome-C shunts oxidation of NADH
NADH-oxidasation mitochondrial activity in hypotension when you block the respiratory chain
Do mitochondria swell much?
Some properties of protomitochondria
Protomitochondria in liver cells
Germinal protomitochondria
Fluorimetric comparison of protomitochondria and mitochondria
Photometry and fluorimetry of protomitochondria from liver of young and adult rats.-Degradation of mitochondria to lipofuscin during heating and lighting
Production of superoxide and lipofuscin after the loss of flavin by NADH-dehydrogenase
Some properties of mitochondrial lipofuscin.