Bookeditors, Michael J. Skvarla, Ronald Ochoa, Jose Carlos Verle Rodrigues and H. Joel Hutcheson.
Summary: This book gathers papers presented at the annual meetings of the Acarological Society of America (ASA), jointly organized with the Entomological Society of America. The ASA plans to publish presentations from its annual meetings on a yearly basis; this book represents the first in the series.
Contents:
Intro; Introduction; Contents; 1 Use of the Ion Torrent PGM for Determining the Genomic Sequences of Francisella and Coxiella-Like Endosymbionts and Rickettsia Directly from Hard Ticks; 1.1 Introduction; 1.2 Materials and Methods; 1.2.1 Tick Collection and Storage, DNA Extraction and Storage; 1.2.2 PCR Testing of Samples for Their Content of CLE, FLE, and Spotted Fever Group Rickettsia; 1.2.3 DNA Sequencing of OmpA Amplicons; 1.2.4 IT-PGM Library Preparation and Sequencing; 1.2.5 Repli-G Procedures; 1.2.6 Bioinformatic Analysis of IT-PGM Sequences; 1.3 Results 1.3.1 Library Preparation and IT-PGM Sequence Reads Obtained1.3.2 Repli-G Enhancement of Read Depth; 1.3.3 Tick Samples with Francisella-Like Endosymbionts (FLE); 1.3.4 Tick Samples with Coxiella-Like Endosymbionts (CLE); 1.3.5 Amblyomma americanum Tick Samples Containing Rickettsia amblyommatis and AamGA-CLE; 1.3.6 Amblyomma maculatum Tick Samples Containing Rickettsia parkeri or R. andeanae and Amac-FLE; 1.3.7 Analysis of Bacterial Sequences Present in the Ticks Dermacentor andersoni and D. occidentalis 1.3.8 Analysis of Dermacentor variabilis from Virginia, Georgia, and Ohio for Rickettsia and FLE1.3.9 Detection of Rickettsia montanensis in Dermacentor variabilis and Rhipicephalus sanguineus from Oklahoma; 1.4 Discussion and Conclusions; 1.4.1 Library Preparation and Sequencing; 1.4.2 Genome Assembly and Annotation; 1.4.3 Limitations of Approach and Possible Remedies; 1.4.4 Value of Partial Symbiont Genome Sequences from the IT-PGM; References; 2 Field Studies in Acarology: Joint Base San Antonio, TX: 2015-2017; 2.1 Introduction; 2.2 Materials and Methods; 2.3 Results; 2.4 Discussion 5.1 Introduction5.2 Materials and Methods; 5.2.1 Genomic DNA Extraction and Pyrosequencing; 5.2.2 Study Organisms, Quality Control of Data and Taxonomic Assignations; 5.2.3 Analysis of Metabolic Function; 5.3 Results and Discussion; 5.3.1 Bacteriome, and Metabolic Predictions; 5.3.2 NGS Fungal Diversity; 5.4 Conclusion; References; 6 Biodiversity of Phytoseiidae (Acari: Mesostigmata) of Annual Specialty Crop Systems: The Current State of Knowledge Worldwide and the Need for Study in North America; 6.1 Methods; 6.2 Results; 6.2.1 Diversity of Phytoseiids in Annual Specialty Crops Worldwide