Bookeditors, Didier Reinhardt and Anil K. Sharma.
Summary: This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation.
Contents:
Intro; Preface; Contents; About the Editors; Part I: Root Symbioses;
1: Synthetic Plasmids to Challenge Symbiotic Nitrogen Fixation Between Rhizobia and Legumes; 1.1 Introduction; 1.1.1 Formation of Effective Nodules on Legume Roots; 1.1.2 Genes of Rhizobia Modulating Nodule Formation and Invasion; 1.1.3 From Specific to Promiscuous Symbioses; 1.1.4 Are Many Keys Responsible for ``Much Harmony?́́; 1.2 A Forward Genetic Approach to Study Nodulation Genes; 1.2.1 pMSy
m2: A Small Replicon That Confers Nodulation to Recipient Bacteria 1.3 pMSy
m2: A Successful Step Towards Constructing Synthetic Symbiotic RepliconsReferences;
2: In Vivo Analysis of Rhizosphere Enzyme Activities by the Use of Plastic Syringes; 2.1 Introduction; 2.2 Description of the Method and Discussion; 2.3 Conclusions and Outlook; References;
3: Characterization of Arbuscular Mycorrhizal Communities in Roots of Vineyard Plants; 3.1 Introduction; 3.2 Approaches, Techniques and Results; 3.2.1 Root Sampling in Vineyard; 3.2.2 DNA Extraction; 3.2.3 Amplification of AMF Phylogenetic Marker 3.2.4 High-Throughput Sequencing, Data Processing and Community Analysis3.3 Discussion/Conclusions; References;
4: Molecular Methods for Research on Actinorhiza; 4.1 Introduction; 4.1.1 Actinorhizal Plants; 4.1.2 The Actinomycete Frankia; 4.1.3 Development of Actinorhizae, the Actinorhizal Nodules; 4.1.4 Specific Issues; 4.2 Approaches, Techniques, and Results; 4.2.1 Plant Side; 4.2.1.1 Growing Actinorhizal Plants; 4.2.1.2 Nodulation Experiments; Nodulation in Hydroponics; Spot Inoculation; Split Root Culture System; 4.2.1.3 Functional Genomics; Nucleic Acid Extraction 4.3 Conclusions and OutlookReferences;
5: Molecular and Functional Characterization of Beneficial Bacteria Associated with AMF Spores; 5.1 Introduction; 5.1.1 Arbuscular Mycorrhizal Fungi; 5.1.2 Bacteria Associated with AMF Spores and Their Functional Roles; 5.2 Approaches, Techniques, and Results; 5.2.1 Fungal Material and Spore Collection; 5.2.2 Culture-Independent Approaches for the Detection of Bacteria Strictly Associated with AMF Spores; 5.2.2.1 Techniques; 5.2.2.2 Results; 5.2.3 Culture-Dependent Approaches for the Quantification of Bacteria Associated with AMF Spores Plant Genomic Resources4.2.1.4 Genetic Transformation of Actinorhizal Plants; Agrobacterium tumefaciens-Mediated Transformation of Actinorhizal Plants (Stable); Agrobacterium rhizogenes-Mediated Transformation of Actinorhizal Plants (Hairy Root); 4.2.1.5 Imaging the Early Stages of Frankia Colonization and Associated Host Responses in Living Tissues; 4.2.2 Bacterial Side; 4.2.2.1 Frankia Cultivation and Isolation; 4.2.2.2 Molecular and ``Omics ́́Tools to Study the Bacterial Partner Frankia; 4.2.3 Molecular Tools Used to Analyze Abiotic Stresses on Actinorhizal Plants