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- Bookeditors, Yi-Wei Tang and Charles W. Stratton.Summary: This newly updated edition of the well informed guide covers the more advanced and less cumbersome methods of analysis that embattled hospital microbiology labs must adopt to replace the slow and cumbersome culture-based assays of pathogenic microorganisms.
Contents:
Automated Blood Cultures / X.Y. Han
Laboratory Automation in Clinical Bacteriology / A. Croxatto
Biochemical Profile-Based Microbial Identification Systems / S. Hafeez and J. Aslanzadeh
Advanced Phenotypic Antimicrobial Susceptibility Testing Methods / C.W. Stratton
Rapid Microbial Antigen Tests / S. Campbell and M.L. Landry
Antibody Detection: Principles and Applications / Y.F. (Wayne) Wang
Procalcitonin and Other Host-Response-Based Biomarkers for Evaluation of Infection and Guidance of Antimicrobial Treatment / P. Schuetz, R. Sager, Y. Wirz and B. Mueller
Functional Assessment of Microbial, Viral, and Parasitic Infections Using Real-Time Cellular Analysis / D. Jin, X. Xu, M. Zheng, A. Mira, B.J. Lamarche and A.B. Ryder
Cellular Fatty Acid-Based Microbial Identification and Antimicrobial Susceptibility Testing / N. Parrish and S. Riedel
MALDI-TOF Mass Spectrometry-Based Microbial Identification and Beyond / A. Mellmann and J. Müthing
Transcriptomic Techniques in Diagnostic Microbiology / Z.E. Holcomb and E.L. Tsalik
The Use of Microbial Metabolites for the Diagnosis of Infectious Diseases / M.J. Thalavitiya Acharige, S.S. Koshy and S. Koo
Nucleic Acid Extraction and Enrichment / J.H. Shin
Nonamplified Probe-Based Microbial Detection and Identification / F. Wu, T. Hong and P. Della-Latta
Molecular Typing Techniques: State of the Art / R.V. Goering
PCR and Its Variations / E.A. Powell and M. Loeffelholz
Non-PCR Amplification Techniques / R.C. She, T.E. Schutzbank and E.M. Marlowe
Real-Time and Digital PCR for Nucleic Acid Quantification / A.J. McAdam
Direct Nucleotide Sequencing for Amplification Product Identification / T. Hong
Solid and Suspension Microarrays for Detection and Identification of Infectious Diseases / S. Dunbar, J. Farhang, S. Das, S. Ali and H. Qian
Real-Time Detection of Amplification Products Through Fluorescence Quenching or Energy Transfer / C. Otto and S. Huang
PCR/Electrospray Ionization-Mass Spectrometry as an Infectious Disease Diagnostic Tool / V. Özenci and K. Strålin
Nucleic Acid Amplicons Detected and Identified by T2 Magnetic Resonance / J.L. Snyder, H.S. Lapp, Z.-X. Luo, B. Manning and T.J. Lowery
Molecular Contamination and Amplification Product Inactivation / S. Sefers and J.E. Schmitz. - ArticleFischbach M, Roubinian JR, Talal N.J Immunol. 1978 Jun;120(6):1856-61.Lipopolysaccharide (LPS) is able to induce autoantibodies reversibly in normal mice. Single or multiple doses of LPS induced a rapid and dose-dependent rise in antibodies to Poly A from 113 ng to 590 ng/ml serum as determined by Millipore filter radioimmunoassay. The response peaked at day 3 and was over by day 21. No response was seen if the LPS was chemically inactivated or injected into genetically nonresponsive mice. Antibodies were specific for Poly A and were not induced by T cell mitogens. Sucrose gradient ultracentrifugation demonstrated only IgM antibody. Neonatal thymectomy altered neither the immunoglobulin class nor quantity of antibody. Neonatal splenectomy did not affect antibody class but reduced the amount produced. No free Poly A could be detected in circulation after LPS stimulation. These findings suggest that normal mice have B lymphocytes capable of limited and reversible autoantibody production.