BookN.S. Punekar.
Summary: This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.
Contents:
Part I: Enzyme catalysis
a perspective
Enzymes -Their place in Biology
Enzymes
Historical aspects
Exploiting enzymes
Technology
On enzyme nomenclature and classification
Hallmarks of an enzyme catalyst
Origins of enzyme catalytic power
Origins of enzyme catalytic power
Which enzyme uses what trick? Some remarks
Structure and catalysis: Conformational flexibility and protein motion
Part II: Enzyme kinetic practice and measurements
Chemical kinetics
Fundamentals
Concepts of equilibrium and steady-state
ES complex and pre-steady-state kinetics
Principles of enzyme assays
Good kinetic practices
Quantification of catalysis and measures of enzyme purity
Henri-Michaelis-Menten equation
More complex rate expressions
Enzyme kinetic data
Collection and analysis
Part III: Elucidation of Kinetic mechanisms
Approaches to kinetic mechanism
Overview
Analysis of initial velocity patterns
Enzyme inhibition analyses
Irreversible inhibitions
Reversible inhibitions
Alternate substrate (product) interactions
pH Studies with enzymes
Isotopes in enzymology
Isotope exchanges at equilibrium
Isotope effects in enzymology
From kinetic data to mechanism and back
Part IV: Chemical mechanisms and catalysis
Chemical reactivity and molecular interactions
Acid-base chemistry and catalysis
Nucleophilic catalysis and covalent reaction intermediates
Phosphoryl group chemistry and importance of ATP
Enzymatic oxidation-reduction reactions
Carboxylations and decarboxylations
Electrophilic catalysis and amino acid transformations
Integrating kinetic and chemical mechanisms
A synthesis
Part V: Frontiers in enzymology
Regulation of enzyme activity
In vitro versus in vivo
Concept and consequences
Future of enzymology
An appraisal
Closure
Whither Enzymology.