BookNitish Kumar editor.
Summary: For the majority of the world's population, medicinal and aromatic plants are the most important source of life-saving drugs. Biotechnological tools represent important resources for selecting, multiplying and conserving the critical genotypes of medicinal plants. In this regard, in-vitro regeneration holds tremendous potential for the production of high-quality plant-based medicines, while cryopreservation - a long-term conservation method using liquid nitrogen - provides an opportunity to conserve endangered medicinal and aromatic plants. In-vitro production of secondary metabolites in plant cell suspension cultures has been reported for various medicinal plants, and bioreactors represent a key step toward the commercial production of secondary metabolites by means of plant biotechnology. Addressing these key aspects, the book contains 29 chapters, divided into three sections. Section 1: In-vitro production of secondary metabolites Section 2: In-vitro propagation, genetic transformation and germplasm conservation Section 3: Conventional and molecular approaches.
Contents:
Intro; Preface; Contents; About the Editor; Part I: In Vitro Production of Secondary Metabolite;
Chapter 1: Production ofPlant Secondary Metabolites: Current Status andFuture Prospects; 1.1 Introduction; 1.1.1 Plant Secondary Metabolites; 1.1.2 Terpenes; 1.1.3 Phenolic Compounds; 1.1.4 Nitrogen-Containing Compounds; 1.2 Production ofPlant Secondary Metabolites; 1.2.1 Callus Culture; 1.2.2 Hairy Root Culture; 1.2.3 Organ Culture; 1.2.4 Elicitation; 1.2.5 Endophytes; 1.2.6 Nitric Oxide; 1.2.7 Abiotic Stress; 1.2.8 Bioreactor; 1.2.9 Metabolic Engineering; 1.2.10 Immobilization 1.3 Production ofValuable Pharmaceutical Compounds ThroughIn Vitro Culture Techniques1.3.1 Taxol; 1.3.2 Morphine andCodeine; 1.3.3 Ginsenosides; 1.3.4 L-DOPA; 1.3.5 Diosgenin; 1.3.6 Capsaicin; 1.3.7 Berberine; 1.3.8 Camptothecin; 1.3.9 Vincristine andVinblastine; 1.4 Conclusions; References;
Chapter 2: The Effects ofrol Genes ofAgrobacterium rhizogenes onMorphogenesis andSecondary Metabolite Accumulation inMedicinal Plants; 2.1 Introduction; 2.2 Effect ofrolA Gene onMorphogenesis; 2.3 Effect ofrolB Gene onMorphogenesis; 2.4 Effect ofrolC Gene onMorphogenesis 2.5 Effect ofrolD Gene onMorphogenesis2.6 Effect ofrolABC Gene onMorphogenesis; 2.7 The 'rol Effect' onSecondary Metabolites inPlants; 2.8 Effect ofrolA Gene onPlant Secondary Metabolite Accumulation; 2.9 Effect ofrolB Gene onPlant Secondary Metabolite Accumulation; 2.10 Effect ofrolC Gene onPlant Secondary Metabolite Accumulation; 2.11 Synergistic Effect ofrol ABC Gene onPlant Secondary Metabolite Accumulation; 2.12 Conclusion; References;
Chapter 3: Conventional andBiotechnological Approaches toEnhance Steviol Glycosides (SGs) inStevia rebaudiana Bertoni; 3.1 Introduction 3.2 Conventional Approaches3.2.1 Enhancement by Physical Factors; 3.2.2 Enhancement by Chemical Factors; 3.3 Biotechnological Interventions; 3.3.1 Micropropagation; 3.3.2 Enhancement by Biological Factors; 3.3.3 Hairy Root Culture andMetabolic Engineering; 3.4 Conclusion; References;
Chapter 4: Effect ofChemical Elicitors onPentacyclic Triterpenoid Production inInVitro Cultures ofAchyranthes aspera L.; 4.1 Introduction; 4.1.1 Taxonomy ofthePlant; 4.1.2 Ethnobotanical Significance; 4.1.3 Pharmacology; 4.1.4 Chemical Properties; 4.2 Methodology; 4.2.1 Sampling andCollection 4.2.2 Sample Authentication4.2.3 Sample Processing; 4.2.4 Effect ofPlant Growth Regulators onShoot Induction andRate ofMultiplication; 4.2.5 Effect ofElicitors withandWithout PGRs forProduction ofTriterpenoids; 4.2.6 Culturing Conditions; 4.2.7 Extraction; 4.2.8 RP-UFLC Analysis ofSelected Triterpenoids; 4.3 Results andDiscussion; 4.3.1 RP-UFLC Analysis ofSelected Triterpenoids; 4.3.2 Use ofSalicylic Acid (SA), Methyl Jasmonate (MeJA), Jasmonic Acid (JA), andChitosan (CH) inMedia Fortified withandWithout PGRs forProduction ofTriterpenoids