BookXu Fang, Yinbo Qu, editors.
Summary: The book provides an overview of the current knowledge on cellulolytic enzymes and their applications. It summarizes the mechanisms of synthesis and hydrolysis of cellulolytic enzymes, industrial fungal strains, genetic engineering of fungal strains and application of cellulolytic enzymes. This book will be a useful reference for researchers and bioengineering experts engaged in lignocelluloses biodegradation, biomass utilization, enzyme production and fungal molecular biology.
Contents:
Intro; Preface; Contents; Contributors;
Chapter 1: Pretreatment Process and Its Synergistic Effects on Enzymatic Digestion of Lignocellulosic Material; 1 Introduction; 2 Lignocellulose Compositions; 3 Pretreatment of Lignocellulosic Materials; 4 Physical Pretreatment; 4.1 Mechanical Pretreatment; 4.2 Pyrolysis; 4.3 Irradiation; 5 Chemical Pretreatment; 5.1 Ozonolysis; 5.2 Acid Pretreatment; 5.3 Alkaline Pretreatment; 5.4 Ionic Liquid Pretreatment; 5.5 Organosolv Pretreatment; 6 Physical-Chemical Pretreatment; 6.1 Steam Explosion; 6.2 Liquid Hot Water Pretreatment; 6.3 Oxidative Pretreatment. 6 Metabolic Engineering of Filamentous Fungi Using CRISPR-Cas9-Based Genome Editing System7 Conclusion and Future Prospects; References;
Chapter 3: Lignocellulase Formation, Regulation, and Secretion Mechanisms in Hypocrea jecorina (Trichoderma reesei) and Other Filamentous Fungi; 1 Introduction; 2 Transcriptional Mechanisms that Regulate Cellulase and Hemicellulase Gene Expression; 2.1 Transcriptional Factors; 2.2 Other Regulatory Factors; 3 Chromatin Remodeling; 4 Transcriptional Regulation of Cellulase and Hemicellulase in H. jecorina by External Signals; 5 Prospects; References. 6.4 SPORL Pretreatment6.5 Ammonia Fiber Explosion (AFEX) and Ammonia Recycle Percolation(ARP); 6.6 CO2 Explosion; 7 Biological Pretreatment; 8 Summary of Biomass Pretreatment Methods; References;
Chapter 2: Metabolic Engineering of Fungal Strains for Efficient Production of Cellulolytic Enzymes; 1 Introduction; 2 Selective Markers for Genetic Engineering of Filamentous Fungi; 3 Promoter Engineering to Improve Cellulase Production; 4 Engineering of Transcription Regulators to Improve Cellulase Production; 5 Improvement of Cellulase Production by Morphology Engineering.
Chapter 4: Development of Highly Efficient, Low-Cost Lignocellulolytic Enzyme Systems in a Penicillium: From Strain Screening to Systems Biology1 Introduction; 2 Genome and Transcriptome Analyses of P. oxalicum Wild-Type Strain 114-2 and Mutant JU-A10-T; 2.1 Global Comparison of Genomes of 114-2 and JU-A10-T; 2.2 Comparative Transcriptome Analysis of 114-2 and JU-A10-T; 3 Secretome Analysis of P. oxalicum Wild-Type Strain 114-2 and Mutant JU-A10-T; 4 Improving Lignocellulolytic Enzyme Production by Redesigning the Regulatory Pathway; 4.1 Transcription Factors.