BookSeiji Masuda, Shingo Izawa, editors.
Summary: The focus of this book is to introduce up-to-date information on applications and practical use of RNA for agriculture, biotechnology and medicine. It provides unique ideas, tools, and methods in detail from a variety of scientific and technical disciplines. RNA science has progressed enormously in recent decades, and vast amounts of information on RNA functions and their regulatory mechanisms are becoming available. Such a progress opened the door to an age of practical application of RNA in many fields including agriculture, plant science, medical science, brewing and fermentation technology, and material production. This book inspires its readership and contributes to not only expansion in application of RNA but also to basic research.
Contents:
Intro; Contents;
Chapter 1: Improvement of Yeast Fermentation Efficiency Utilizing mRNAs Preferentially Translated Under Translational Repression; 1.1 Introduction; 1.2 Translational Repression and Formation of Cytoplasmic mRNP Granules; 1.3 HSP26 Is Preferentially Translated Under Glucose Depletion; 1.4 Translational Repression and Induction of mRNP Granule Formation Due to Ethanol Stress; 1.5 BTN2 Is Preferentially Translated Under Translation Repression Due to High Concentrations of Ethanol; 1.6 Improve Ethanol Tolerance and Fermentability by BTN2 Promoter. 1.7 ADH7 and BDH2 Are Preferentially Translated Under Translational Repression Due to Vanillin1.8 Improvement of Tolerance Against Biomass Conversion Inhibitors Using ADH7 Promoter; 1.9 Characteristics of the Promoters that Enable the Preferential Translation Under the Repression of Overall Protein Synthesis; 1.10 Conclusion; References;
Chapter 2: Constructing Mutant Ribosomes Containing Mutant Ribosomal RNAs; 2.1 Function and Structure of the Ribosome; 2.2 Constructing Mutant Ribosomes Containing Modified rRNAs; 2.2.1 The Use of Non-model Microorganisms. 5.2.5 In Vitro Selection for Structure-Switching Signaling Aptamers5.2.6 Other Types of Modified SELEX; 5.2.6.1 Modified SELEX Using Improved Libraries; 5.2.6.2 Modified SELEX with Improved Aptamer Separation Process; 5.2.6.3 Modified SELEX for Cell Surface or Whole-Cell Targets; 5.3 Biosensor Application of Aptamers for Natural Toxins; 5.3.1 Sensor Types; 5.3.2 Aptasensors for Natural Toxins; 5.4 Conclusions and Future Prospects; References;
Chapter 6: Rational Design of Artificial Riboswitches; 6.1 Introduction; 6.2 Research Initiation; 6.3 Bacterial ON-Riboswitches Modulating mRNA Cleavage.
Chapter 4: Reprogramming of Cells by Lactic Acid Bacteria4.1 Introduction; 4.2 Microbial Communities; 4.3 Modulation of Cellular Homeostasis, Cell Lineage Fate, and Stem Cell Development by Endosymbiotic Microbial Community; 4.4 Cell Reprogramming by Lactic Acid Bacteria (LAB); 4.5 Cell Reprogramming by Members of Mycobacteriaceae; 4.6 Conclusions; References;
Chapter 5: Generation of Aptamers Against Natural Toxins and Their Application as Biosensors; 5.1 Introduction; 5.2 Strategies for Aptamer Generation; 5.2.1 Classic SELEX; 5.2.2 Allosteric Selection; 5.2.3 CE-SELEX; 5.2.4 GO-SELEX.