BookDhiraj Kumar, Chengliang Gong, editors.
Summary: This book provides an overview on the basics in insect molecular biology and presents the most recent developments in several fields such as insect genomics and proteomics, insect pathology and applications of insect derived compounds in modern research. The book aims to provide a common platform for the molecular entomologist to stimulate further research in insect molecular biology and biotechnology. Insects are one of the most versatile groups of the animal kingdom. Due to their large population sizes and adaptability since long they attract researchers' interest as efficient resource for agricultural and biotechnological purposes. Several economically important insects such as Silkworms, Honey Bee, Lac and Drosophila or Termites were established as invertebrate model organisms. Starting with the era of genetic engineering, a broad range of molecular and genetic tools have been developed to study the molecular biology of these insects in detail and thus opened up a new horizon for multidisciplinary research. Nowadays, insect derived products are widely used in biomedical and biotechnology industries. The book targets researchers from both academia and industry, professors and graduate students working in molecular biology, biotechnology and entomology.
Contents:
Intro; Preface; Acknowledgements; Contents; About the Editors; Part I: Insect Genomics and Proteomics;
1: Molecular Marker-Assisted Selection Breeding in Silkworm, Bombyx mori; 1.1 Introduction; 1.2 Quantitative Trait Locus (QTL); 1.3 Reverse Genetics; 1.4 Genome Annotation; 1.5 Extraction of DNA; 1.6 Cutting DNA into Small Fragments; 1.7 DNA Sequencing; 1.7.1 The Maxam-Gilbert technique; 1.7.2 The Sanger dideoxy DNA sequencing technique; 1.7.3 Pyrosequencing; 1.7.4 Shotgun Sequencing; 1.7.5 Whole-Genome Shotgun Sequencing; 1.8 Gene Libraries; 1.9 Preparation of cDNA. 1.10 DNA Fingerprinting (or Genetic Fingerprinting or DNA Testing or DNA Typing or DNA Profiling)1.11 Protection of DNA Fragments; 1.12 Contig; 1.13 Expressed Sequence Tag (EST); 1.14 Transposons; 1.15 Cloning; 1.15.1 Identification of Transformed Cell; 1.16 DNA Probe; 1.17 Blotting; 1.17.1 The Southern Blot; 1.17.2 The Northern Blot; 1.17.3 The Western Blot (Immunoblot); 1.17.3.1 Tissue Preparation; 1.17.3.2 Protein Sample Preparation; 1.17.3.3 Separation of Protein Fractions; 1.17.3.4 Two-Step Method; 1.17.3.5 One-Step Method; 1.17.3.6 Colorimetric Detection. 1.17.3.7 Chemiluminescence1.17.3.8 Radioactive Detection; 1.17.3.9 Fluorescent Detection; 1.18 Polymorphism in Repeated Sequences; 1.19 DNA Markers; 1.19.1 Types of Markers; 1.19.1.1 RFLP; 1.19.1.2 PCR Based Markers; RAPD; AFLP; Minisatellite; Microsatellites: STR, SSR, or SSLPs; Development of Microsatellite Primers; SNP (Single Nucleotide Polymorphism); STS (Sequence Tagged Sites); SCAR (Sequence Characterized Amplified Region); CAPS (Cleaved Amplified Polymorphic Sequence); ISSRs (Inter-Simple Sequence Repeats); DArT (Diversity Array Technology); LCN-DNA (Low Copy Number DNA). 1.20 Markers in Silkworm, Bombyx moriReferences;
2: Molecular, Neuronal, and Behavioral Mechanism of Communication Among Insect Species: A Review; 2.1 Introduction; 2.2 Necessity of Communication; 2.3 Types of Communications Among Insect Species; 2.3.1 Visual Communication; 2.3.2 Tactile and Behavioral Communication; 2.3.3 Acoustic Communication; 2.3.4 Chemical Communication; 2.4 Insect Hydrocarbons and Chemical Communication; 2.5 Neuronal Basis of Insect Communication; 2.6 Molecular and Biochemical Basis of Chemical Communication. 2.7 Pheromones in Communication Among Insect Partners2.8 Molecular Mechanism of Odor Reception in Insects; 2.9 Pheromone Detection by Olfactory Systems; 2.10 Behavioral Mechanism of Communication Among Insect Species; 2.11 Honeybee Dancing; 2.12 Magnetic Orientation; References;
3: Monocyclic Aromatic Hydrocarbons (MAHs) Induced Toxicity in Drosophila: How Close How Far?; 3.1 Introduction; 3.2 Benzene; 3.3 Xylene, Toluene and Styrene; 3.4 Cytotoxicity Induced by Individual of Benzene, Toluene and Xylene on Drosophila.