Bookedited by Benjamin A. Garcia.
Contents:
Crosstalk between cellular metabolism and histone acetylation / Sophie Trefely, Mary T. Doan, and Nathaniel W. Snyder
Purification and enzymatic assay of class I histone deacetylase enzymes / Mark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer, and Michael P. Washburn
Multiplexed quantitative phosphoproteomics of cell line and tissue samples / Johannes Kreuzer, Amanda Edwards, and Wilhelm Haas
Differentiation of peptide isomers and epimers by radical-directed dissociation / Tyler R. Lambeth and Ryan R. Julian
Biochemical analysis of protein arginylation / Junling Wang, John R. Yates III, and Anna Kashina
Site-specific determination of lysine acetylation stoichiometries on the proteome-scale / Yue Chen and Yunan Li
RNA modifications and the link to human disease / Amber Yanas and Kathy Fange Liu
Isolation and characterization of glycosylated neuropeptides / Yang Liu, Qinjingwen Cao, and Lingjun Li
Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins / Igor Maksimovic, Devin Ray, Qingfei Zheng, and Yael David
Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics / Fangxu Sun and Ronghu Wu
Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists / Sourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula, and Mia L. Huang
Biochemical and structural analysis of N-terminal acetyltransferases / Leah Gottlieb and Ronen Marmorstein
Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry / Peng Li, Yuanli Zhen, and Yonghao Yu
Analysis of the topology of ubiquitin chains / Lucia Geis-Asteggiante, Amanda E. Lee, and Catherine Fenselau
Analysis of cardiac troponin proteoforms by top-down mass spectrometry / Timothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin, and Ying Ge
Assays for tyrosine phosphorylation in human cells / Monica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang, and Laurie L. Parker
Metabolomics analysis of lipid metabolizing enzyme activity / Timothy B. Ware, Myungsun Shin, and Ku-Lung Hsu
Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitation / Nestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu, and Christopher M. Overall
Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes / Jeremy D. Osko and David W. Christianson
Quantitative analysis of global protein lysine methylation by mass spectrometry / Peder J. Lund, Stephanie M. Lehman, and Benjamin A. Garcia
The roles of S-nitrosylation and S-glutathionylation in Alzheimer's disease / Ryan R. Dyer, Katarena I. Ford, Renã A.S. Robinson
Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes / Kyle Mohler and Jesse Rinehart
Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes / Nicholas J. Porter and David W. Christianson
Methods for characterizing protein acetylation during viral infection / Laura A. Murray, Ashton N. Combs, Pranav Rekapalli, and Ileana M. Cristea.