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  • Book
    Pranab Dey.
    Summary: This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. In addition, it covers various advanced laboratory techniques, such as immunocytochemistry, flow cytometry, liquid based cytology, polymerase chain reaction, tissue microarray, and molecular technology. Further, the book includes numerous color illustrations, tables and boxes to familiarize the reader with the work of a pathology laboratory. The book is mainly intended for postgraduate students and fellows in pathology as well as practicing pathologists. The book is also relevant for all the laboratory technicians and students of laboratory technology.

    Fixation of histology samples
    Processing of tissue
    Embedding of tissue
    Decalcification of bone
    Tissue microtomy
    Frozen section of tissue: technique and applications
    Staining principle and general procedure of stain
    Haematoxylin and eosin stain
    Special stains for protein, lipid, carbohydrate, nucleic acid and pigments
    Stains for the connective tissue
    Stains for the amyloid
    Stains for the microbial organisms
    Cytology sample: procurement, fixation and processing
    Routine staining in cytology laboratory
    Fine needle aspiration biopsy technique
    Immunochemistry in histology and cytology: principle, technique and applications
    Flow cytometry: principle, technique and applications
    Digital image analysis and virtual microscopy: principle, technique and applications
    Liquid based cytology and automated screening devices
    Polymerase chain reaction and DNA sequencing: principle, technique and applications
    Fluorescent in situ hybridisation techniques: principle, technique and applications
    Tissue microarray: technique and applications
    Sanger sequencing and next generation gene sequencing: basic principles and applications
    Light microscope: optical components, principle and handling
    Fluorescence microscope and confocal microscopy: basic parts and principle
    Laboratory organization and quality control
    Laboratory safety: essential precautions.
    Digital Access Springer 2018