BookPranab Dey.
Summary: This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. In addition, it covers various advanced laboratory techniques, such as immunocytochemistry, flow cytometry, liquid based cytology, polymerase chain reaction, tissue microarray, and molecular technology. Further, the book includes numerous color illustrations, tables and boxes to familiarize the reader with the work of a pathology laboratory. The book is mainly intended for postgraduate students and fellows in pathology as well as practicing pathologists. The book is also relevant for all the laboratory technicians and students of laboratory technology.
Contents:
Fixation of histology samples
Processing of tissue
Embedding of tissue
Decalcification of bone
Tissue microtomy
Frozen section of tissue: technique and applications
Staining principle and general procedure of stain
Haematoxylin and eosin stain
Special stains for protein, lipid, carbohydrate, nucleic acid and pigments
Stains for the connective tissue
Stains for the amyloid
Stains for the microbial organisms
Cytology sample: procurement, fixation and processing
Routine staining in cytology laboratory
Fine needle aspiration biopsy technique
Immunochemistry in histology and cytology: principle, technique and applications
Flow cytometry: principle, technique and applications
Digital image analysis and virtual microscopy: principle, technique and applications
Liquid based cytology and automated screening devices
Polymerase chain reaction and DNA sequencing: principle, technique and applications
Fluorescent in situ hybridisation techniques: principle, technique and applications
Tissue microarray: technique and applications
Sanger sequencing and next generation gene sequencing: basic principles and applications
Light microscope: optical components, principle and handling
Fluorescence microscope and confocal microscopy: basic parts and principle
Laboratory organization and quality control
Laboratory safety: essential precautions.