Bookedited by Nigel Scrutton.
Contents:
Section I. Enzyme discovery. Enzyme discovery : enzyme selection and pathway design / Pablo Carbonell, Mathilde Koch, Thomas Duigou, and Jean-Loup Faulon
Section II. Enzyme engineering tools and platforms. Cell-free synthetic biology for pathway prototyping / Ashty S. Karim and Michael C. Jewett ; Fast and flexible synthesis of combinatorial libraries for directed evolution / Joanna C. Sadler, Lucy Green, Neil Swainston, Douglas B. Kell, and Andrew Currin
Section III. Enzyme families : parts and platforms for chemical diversity. Sesquiterpene synthase-catalyzed conversion of a farnesyl diphosphate analogue to a nonnatural terpenoid ether / Florence Huynh, David J. Miller, and Rudolf K. Allemann ; In vivo platforms for terpenoid overproduction and the generation of chemical diversity / Guangkai Bian, Tian Ma, and Tiangang Liu ; Imine reductases, reductive aminases, and amine oxidases for the synthesis of chiral amines : discovery, characterization, and synthetic applications / Sebastian C. Cosgrove, Agata Brzezniak, Scott P. France, Jeremy I. Ramsden, Juan Mangas-Sanchez, Sarah L. Montgomery, Rachel S. Heath, and Nicholas J. Turner ; Experimental protocols for generating focused mutant libraries and screening for thermostable proteins / Max J.L.J. Fürst, Caterina Martin, Nikola Lončar, Marco W. Fraaije ; Characterization of cytochrome P450 enzymes and their applications in synthetic biology / Laura N. Jeffreys, Hazel M. Girvan, Kirsty J. McLean, and Andrew W. Munro
Section IV. Genome engineering for enzyme pathways. Metabolic engineering of Saccharomyces cerevisiae using a trifunctional CRISPR/Cas system for simultaneous gene activation, interference, and deletion / Carl Schultz, Jiazhang Lian, and Huimin Zhao ; YeastFab : high-throughput genetic parts construction, measurement, and pathway engineering in yeast / Eva Garcia-Ruiz, Jamie Auxillos, Tianyi Li, Junbiao Dai, and Yizhi Cai
Section V. Bioengineered strains with designed enzyme pathways. Halomonas and pathway engineering for bioplastics production / Jiang Xiao-Ran, Yin Jin, Chen Xiangbin, and Chen Guo-Qiang ; An engineering approach for rewiring microbial metabolism / Sebastian Wenk, Oren Yishai, Steffen N. Lindner, and Arren Bar-Even ; Multifragment DNA assembly of biochemical pathways via automated ligase cycling reaction / Christopher J. Robinson, Mark S. Dunstan, Neil Swainston, James Titchmarsh, Eriko Takano, Nigel S. Scrutton, and Adrian J. Jervis ; Commodity chemicals from engineered modular type I polyketide synthases / Satoshi Yuzawa, Amin Zargar, Bo Pang, Leonard Katz, and Jay D. Keasling ; Modular pathway rewiring of yeast for amino acid production / Quanli Liu, Tao Yu, Kate Campbell, Jens Nielsen, and Yun Chen.