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  • Book
    edited by Vicky Chapman, Cathy Charles.
    Contents:
    Labour and normal birth / Cathy Charles
    Vaginal examinations and amniotomy / Vicky Chapman
    Fetal heart rate monitoring in labour / Bryony Read
    Perineal trauma and suturing / Vicky Chapman
    Examination of the newborn baby at birth / Caroline Rutter
    Home birth / Cathy Charles
    Water for labour and birth / Cathy Charles
    Malpositions and malpresentations in labour / Vicky Chapman
    Slow progress in labour / Vicky Chapman
    Assisted birth: ventouse and forceps / Cathy Charles
    Caesarean section / Cathy Charles
    Vaginal birth after caesarean section / Vicky Chapman
    Preterm birth / Charlise Adams
    Breech birth / Lesley Shuttler
    Twins and higher-order births / Jo Coggins
    Obstetric haemorrhage / Hannah Bailey
    Emergencies in labour and birth / Hannah Bailey
    Neonatal and maternal resuscitation / Nick Castle
    Induction of labour / Cathy Charles
    Pre-eclampsia and diabetes / Annette Briley
    Stillbirth and neonatal death / Cathy Charles
    Risk management, litigation and complaints / Cathy Charles
    Intrapartum blood tests / Vicky Chapman
    Medicines and the midwife / Vicky Chapman.
    Digital Access Wiley 2017
  • Article
    Normark S, Edlund T, Grundström T, Bergström S, Wolf-Watz H.
    J Bacteriol. 1977 Dec;132(3):912-22.
    Escherichia coli K-12 ampicillin-resistant mutants hyperproducing chromosomal beta-lactamase arose spontaneously from strains carrying ampA1 ampC(+). Such mutants were found even in a recA background. Two Amp(r)-100 strains were analyzed genetically. The Amp(r)-100 resistance level of both strains could be transduced by direct selection for ampicillin resistance. Several classes of ampicillin-resistant transductants were found that differed from one another in the beta-lactamase activity and the ampicillin resistance mediated by an ampA1 ampC(+)-carrying strain. The data suggested that beta-lactamase hyperproduction was due to repetitions of the chromosomal amp genes. The size of the repeated region was calculated from cotransduction estimates, using the formula of Wu (Genetics 54:405-410, 1966), and was found to be about 1 min in one strain and 1.5 min in the other. Second-step Amp(r)-400 mutants were isolated from an Amp(r)-100 strain. The resistance of these mutants was apparently also due to repetitions, each mediating a resistance to about 10 mug/ml. Mutants of wild-type strains that were moderately resistant to ampicillin also gave rise to intermediate-resistance classes, suggesting repetitions of the wild-type amp alleles. F' factors hyperproducing chromosomal beta-lactamase by gene repetitions were constructed. They mediated levels of ampicillin resistance comparable to that of naturally occurring resistance plasmids. The expression of beta-lactamase hyperproduction was not affected by the presence of ampA and ampC alleles in trans and did not act in trans on the other alleles.
    Digital Access Access Options