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- Bookedited by David K. Gardner, Carlos Simón.Contents:
IVF : the first four decades / Mae Wu Healy, Micah J. Hill, and Alan DeCherney
Evaluation and preparation of the infertile couple for in vitro fertilization / David R. Meldrum
Carrier screening / Stephanie Hallam and Gregory Porreca
Testing ovarian reserve / Daniela Galliano, Nuria Pellicer, and Antonio Pellicer
Basics of ovarian stimulation / Sandra J. Tanahatoe, Frank J.M. Broekmans, and Bart C.J.M. Fauser
Role of antagonists in human IVF : the tool to a safer and more patient friendly IVF / Evangelos Papanikolaou and Robert Najdecki
Ultrasonography in IVF / Roger A. Pierson
Oocyte retrieval / Nina Resetkova and Michel M. Alper
In vitro maturation for clinical use / Melanie L. Walls and Roger Hart
Sperm preparation for IVF and ICSI / Christopher J. De Jonge and Nancy Bossert
Assisted fertilization / Zsolt Peter Nagy
Analysis of fertilization / Basak Balaban
Human embryo development and assessment of viability / David K. Gardner and Markus Montag
Embryo culture systems / David K. Gardner and Michelle Lane
Polar body, cleavage stage and trophectoderm biopsy / Antonio Capalbo, Laura Rienzi, Danilo Cimadomo, and Filippo Maria Ubaldi
Preimplantation genetic testing / Nathan R. Treff and Carmen Rubio
Oocytes and embryo cryopreservation / Ana Cobo
Quality control and quality assurance in the IVF laboratory / David Mortimer and Sharon T. Mortimer
Oocyte donation / Irene Woo and Richard J. Paulson
Endometrial receptivity / Sujata Lalitkumar, Eva Gomez, Maria Ruiz-Alonso, Diana Valbuena, Felipe Vilella, and Carlos Simón
Embryo transfer / Giulia Mariani and Jose Bellver
Multiple vs singleton births : consequences and prevention / Ernesto Bosch
Monitoring and evaluation of infertility treatments : is there a priority for monitoring of cognitive function in children born from ART and other treatments? / Michael J. Davies, Vivienne M. Moore.Digital Access TandFonline 2017 - ArticleCherny DI, Aleksandrov AA, Zarudnaya MI, Kosaganov YN, Lazurkin YS, Beabealashvilli RS, Svochkina LP.Eur J Biochem. 1977 Sep 15;79(1):309-17.The complexes of T2 DNA with RNA polymerase of Escherichia coli were studied by two methods: kinetic formaldehyde method with preliminary fixation of complexes with low formaldehyde concentrations, and electron microscopy. For electron-microscopic investigations the effect of different conditions of formaldehyde fixation for DNA-RNA-polymerase complexes was studied and optimal fixation conditions were found. The suggested fixation method for DNA-RNA-polymerase complexes allows investigation of RNA polymerase molecule distribution on DNA in a wide range of conditions (ionic strength of the solution, weight ration of enzyme to DNA etc.). The comparison of the concentration of RNA polymerase molecules bound to DNA, determined by electron microscopy, and the concentration of defects in DNA as determined by the kinetic formaldehyde method, showed their coincidence. The electron-microscopic procedure was used to make maps of RNA polymerase distribution on T7 DNA. A correlation between the binding regions of the enzyme and the genetic map of early DNA T7 region was found.