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  • Book
    [edited by] Valter Tucci.
    Contents:
    1. Genetic screens in neurodegeneration
    2. Computational epigenomics
    3. Behavioral phonetyping in zebrafish: the first models of alcohol induced abnormalities
    4. How does stress affect energy balance?
    5. Interactions of experience-dependent plasticity and LTP in the hippocampus during associative learning
    6. The genetics of cognition in schizophrenia: combining mouse and human studies
    7. The biological basis of economic choice
    8. Interval-timing protocols and their relevancy to the study of temporal cognition and neurobehavioral genetics
    9. Toolkits for cognition: from core knowledge to genes
    10. Quantitative genetics of behavioral phenotypes
    11. Behavioral phenotyping in genetic mouse models of autism spectrum disorders: a translational outlook
    12. Genetics of human sleep and sleep disorders
    13. The endocannabinoid system in the control of behavior
    14. Epigenetics in brain development and disease
    15. Impact of postnatal manipulations on offspring development in rodents
    16. Exploring the roles of genetics and the epigenetic mechanism DNA methylation in honey bee (Apis Mellifera) behavior
    17. Genetics and neuroepigenetics of sleep
    18. Behavioral phenotyping using optogenetic technology
    19. Phenotyping sleep: beyond EEG
    20. A cognitive neurogenetics screening system with a data-analysis toolbox
    21. Mapping the connectional architecture of the rodent brain with fMRI
    22. Cutting edge approaches for the identification and the functional investigation of miRNAs in brain science
    Index.
    Digital Access Wiley 2017
  • Article
    Sato T, Ito K, Yura T.
    Eur J Biochem. 1977 Sep;78(2):557-67.
    Protein compositions of the inner and outer membranes of Escherichia coli K-12 have been analyzed by two-dimensional gel electrophoresis in which proteins are separated according to apparent isoelectric point (first dimension) and to apparent molecular weight (second dimension). Membrane proteins except for a pair of major outer membrane proteins (proteins Ia and Ib) were found to be solubilized effectively by lysis buffer containing urea, Triton X-100, ampholines and 2-mercaptoethanol. The latter two proteins could be solubilized after precipitation of membrane fraction with trichloroacetic acid; they formed a pair of spots at an acidic region on the electropherogram. Another major protein of the outer membrane, protein II, was also identified. Most of the inner and outer membrane proteins were shown to be focused at a pH range between 4 and 6.5. Specific protein patterns characteristic for both the inner and outer membranes could thous be visualized by the present system. At least 120 and 50 protein species were detected for the inner and outer membranes, respectively.
    Digital Access Access Options