Bookedited by Michael E. Harris and Vernon E. Anderson.
Contents:
1. Binding isotope effects for interrogating enzyme-substrate interactions
2. Chemical ligation and isotope labeling to locate dynamic effects
3. Measurement of enzyme isotope effects
4. Extracting kinetic isotope effects from a global analysis of reaction progress curves
5. Natural isotope abundance in metabolites: techniques and kinetic isotope effect measurement in plant, animal and human tissues
6. Measurement of kinetic isotope effects in an enzyme-catalyzed reaction by continuous-flow mass spectrometry
7. Primary deuterium kinetic isotope effects from product yields: rationale, implementation, and interpretation
8. Measurement and prediction of chlorine kinetic isotope effects in enzymatic systems
9. Kinetic deuterium isotope effects in cytochrome P450 reactions
10. Use of isotopes and isotope effects for investigations of diiron oxygenase mechanisms
11. Characterization of substrate, cosubstrate, and product isotope effects associated with enzymatic oxygenations of organic compounds based on compound-specific isotope analysis
12. pH-free measurement of relative acidities, including isotope effects
13. Isotope ratio monitoring ¹³C nuclear magnetic resonance spectrometry for the analysis of position-specific isotope ratios
14. Applications of isotope ratio mass spectrometry in sports drug testing accounting for isotope fractionation in analysis of biological samples
15. Kinetic isotope effect analysis of RNA 2'-O-Transphosphorylation
16. Theory and application of the relationship between steady-state isotope effects on enzyme intermediate concentrations and net rate constants
17. Determining carbon kinetic isotope effects using headspace analysis of evolved CO₂
18. Kinetic isotope effect studies to elucidate the reaction mechanism of RNA-modifying enzymes
19. Measurement of kinetic isotope effects by continuously monitoring isotopologue ratios using NMR spectroscopy
Author index
Subject index.