BookAyaz Najafov, Gerta Hoxhaj.
Summary: Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types.
Contents:
Chapter 1. Introduction
Chapter 2. Procedure
Chapter 3. Good practices
Chapter 4. Optimization and troubleshooting
Chapter 5. Tips and tricks
Chapter 6. Special cases
Chapter 7. Data analysis, storage, retrieval
Appendix A. Buffers and solutions
Appendix B. SDS-PAGE gel tables
Appendix C. SDS-PAGE protocol
Appendix D. Wet transfer and immunoblotting protocol
Appendix E. Home-made enhanced chemiLuminescence (ECL) detection
Appendix F. Stripping protocols
Appendix G. Coomassie staining protocol
Appendix H. Lysis of cells using native conditions
Appendix I. Quick denaturing lysis protocol
Appendix J. Protein tags
Appendix K. Covalent crosslinking of antibodies to beads
References.