Bookedited by Maria Spies and Yann R. Chemla.
Contents:
Chapter 1. Direct fluorescent imaging of translocation and unwinding by individual DNA helicases
Chapter 2. Single-molecule imaging with one color fluorescence
Chapter 3. Measuring membrane protein dimerization equilibrium in lipid bilayers by single-molecule fluorescence microsopy
Chapter 4. Fluorescent labeling of proteins in whole cell extracts for single-molecule imaging
Chapter 5. Quantifying the assembly of multicomponent molecular machines by single-molecule total internal reflection fluorescence microscopy
Chapter 6. How to measure separations and angles between intramolecular fluorescent markers
Chapter 7. Precisely and accurately inferring single-molecule rate constants
Chapter 8. Quantification of functional dynamics of membrane proteins reconstituted in nanodiscs membranes by single turnover functional readout
Chapter 9. Putting Humpty-Dumpty together: clustering the functional dynamics of single biomolecular machines such as the splicosome
Chapter 10. Single-molecule FRET to measure conformational dynamics of DNA mismatch repair proteins
Chapter 11. Single-molecule confocal FRET microscopy to dissect conformational changes in the catalytic cycle of DNA topoisomerases
Chapter 12. Probing the conformational landscape of DNA polymerases using diffusion-based single-molecule FRET
Chapter 13. Methods for investigating DNA accessibility with single nucleosomes
Chapter 14. Single-molecule fluorescence studies of fast protein folding
Chapter 15. Single-molecule multicolor FRET assay for studying structural dynamics of biomolecules
Chapter 16. A multicolor single-molecule FRET approach to study protein dynamics and interactions simultaneously
Chapter 17. Interferometric scattering microscopy for the study of molecular motors
Chapter 18. Enzyme kinetics in femtoliter arrays
Author index
Subject index.