Bookedited by Anna S. Kashina.
Contents:
Protein arginylation: over 50 years of discovery / Anna S. Kashina
Recollection of how we came across the protein modification with amino acids by aminoacyl trna-protein transferase / Hideko Kaji and Akira Kaji
Arginyltransferase: a personal and historical perspective / Ricahrd L. Soffer
Arginylation in a partially purified fraction of 150k x g supernatants of axoplasm and injured vertebrate nerves / Nicholas A. Ingoglia
Preparation of ATE1 enzyme from native mammalian tissues / Anna S. Kashina
Correlated measurement of endogenous ATE1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging / Hideko Kaji and Akira Kaji
Assaying the posttranslational arginylation of proteins in cultured cells / Mauricio R. Galiano and Marta E. Hallak
Assaying ATE1 activity in yeast by [beta]-Gal degradation / Anna S. Kashina
Bacterial expression and purification of recombinant arginyltransferase (ATE1) and arg-tRNA synthetase (RRS) for arginylation assays / Junling Wang and Anna S. Kashina
Assaying ATE1 activity in vitro/ Junling Wang and Anna S. Kashina
High-throughput arginylation assay in microplate format / Sougata Sha, Junling Wang and Anna S. Kashina
Assay of arginyltransferase activity by a fluorescent HPLC method / Koichi Takao
Identification of arginylated proteins by mass spectrometry / Anna S. Kashina and Jon R. Yates III
Analysis of arginylated peptides by subtractive edman degradation / Anna S. Kashina and Jon R. Yates III
Transferase-mediated labeling of protein N-termini with click chemistry handles / Anne M. Wagner [and four others]
Applying arginylation for bottom-up proteomics / H. Alexander Ebhardt
Development of new tools for the studies of protein arginylation / Anna S. Kashina.