Bookedited by Natalia A. Riobo.
Contents:
Luciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells / Silvia Pandolfi and Barbara Stecca
Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / Pawel Niewiadomski and Rajat Rohatgi
Quantitative immunoblotting of endogenous hedgehog pathway components / Shohreh F. Farzan and David J. Robbins
Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi
Rapid screening of Gli2/3 mutants using the Flp-In system / Pawel Niewiadomski and Rajat Rohatgi
Insights into gli factors ubiquitylation methods / Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio
Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri
Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A.
Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo
Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.