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  • Book
    edited by Mathieu Rederstorff.
    Contents:
    Small non-coding RNAs : a quick look in the rearview mirror / Guillaume Clerget, Yoann Abel, and Mathieu Rederstorff
    Alcoholic precipitation of small non-coding RNAs / Guillaume Clerget, Valérie Bourguignon-Igel, and Mathieu Rederstorff
    Quantification and quality control of a small non-coding RNA preparation / Virginie Marchand and Christiane Branlant
    Impact of RNA isolation protocols on RNA detection by Northern blotting / Katrin Damm [and seven others]
    Improved Northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes / Katrin Damm [and seven others]
    Direct cloning of double-stranded RNAs / Manli Shen [and three others]
    Detection and labeling of small non-coding RNAs by splinted ligation / Gabrielle Bourgeois [and three others]
    Fluorescence in situ hybridization of small non-coding RNAs / Valentin Vautrot [and three others]
    RT-qPCR-based quantification of small non-coding RNAs / Fjoralba Zeka, Pieter Mestdagh, and Jo Vandesompele
    Stem-loop RT-PCR based quantification of small non-coding RNAs / Véronique Salone and Mathieu Rederstorff
    miR-RACE : an effective approach to accurately determine the sequence of computationally identified miRNAs / Chen Wang and Jinggui Fang
    Probing small non-coding RNAs structures / Jean-Vincent Philippe [and three others]
    cDNA library generation for the analysis of small RNAs by high-throughput sequencing / Jennifer Gebetsberger, Roger Fricker, and Norbert Polacek
    CLIP-Seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells / Jérôme Saulière and Hervé Le Hir
    Microarray analysis of small non-coding RNAs / Michael Karbiener and Marcel Scheideler
    RML-RACE, PPM-RACE, and qRT-PCR : an integrated strategy to accurately validate miRNA target genes / Chen Wang and Jinggui Fang
    Dual luciferase gene reporter assays to study miRNA function / Thomas Clément, Véronique Salone, and Mathieu Rederstorff
    Gene expression knockdown by transfection of siRNAs into mammalian cells / Yoann Abel and Mathieu Rederstorff
    Efficient and selective knockdown of small non-coding RNAs / Xue-Hai Liang, Wen Shen, and Stanley T. Crooke
    Cell-SELEX : in vitro selection of synthetic small specific ligands / Helena Dickinson [and three others]
    Small non-coding RNAs and aptamers in diagnostics and therapeutics / Marissa Leonard, Yijuan Zhang, and Xiaoting Zhang.
    Digital Access Springer 2015
  • Article
    Kraft LA, Foley CW, Howarth B, Blum MS, Johnson AD.
    Comp Biochem Physiol B. 1978;60(3):233-8.
    1. The patterns of 14CO2 evolution from specifically labeled glucose substrates by washed bull, ram, boar, rabbit, dog, rooster and turkey spermatozoa were similar and indicated the Embden-Meyerhof and Kreb's cycle pathways as the major route of energy metabolism. 2. Honey bee spermatozoa metabolized glucose-3,4-[14C], glucose-[U-14C] or fructose-[U-14C], but not glucose-1-[14C], glucose-2-[14C]or glucose-6-[14C], indicating the presence of the glycolytic pathway, but the absence of respiration via the Kreb's cycle. 3. The rate of glycolysis exceeded the rate of respiration in the spermatozoa of all the species studied. 4. A preferential utilization of glucose-1-[14C] over glucose-6-[14C] was evident in some sperm samples, but no consistent indication of pentose cycle metabolism was observed, due to considerable variability between samples within each group. 5. Fructose metabolism was greater than glucose metabolism in the rooster, less in the dog, boar and turkey, and similar in the spermatozoa from the other species examined. 6. Only ram and bull spermatozoa metabolized acetate-1-[14C] to any extent.
    Digital Access Access Options