Bookedited by Mathieu Rederstorff.
Contents:
Small non-coding RNAs : a quick look in the rearview mirror / Guillaume Clerget, Yoann Abel, and Mathieu Rederstorff
Alcoholic precipitation of small non-coding RNAs / Guillaume Clerget, Valérie Bourguignon-Igel, and Mathieu Rederstorff
Quantification and quality control of a small non-coding RNA preparation / Virginie Marchand and Christiane Branlant
Impact of RNA isolation protocols on RNA detection by Northern blotting / Katrin Damm [and seven others]
Improved Northern blot detection of small RNAs using EDC crosslinking and DNA/LNA probes / Katrin Damm [and seven others]
Direct cloning of double-stranded RNAs / Manli Shen [and three others]
Detection and labeling of small non-coding RNAs by splinted ligation / Gabrielle Bourgeois [and three others]
Fluorescence in situ hybridization of small non-coding RNAs / Valentin Vautrot [and three others]
RT-qPCR-based quantification of small non-coding RNAs / Fjoralba Zeka, Pieter Mestdagh, and Jo Vandesompele
Stem-loop RT-PCR based quantification of small non-coding RNAs / Véronique Salone and Mathieu Rederstorff
miR-RACE : an effective approach to accurately determine the sequence of computationally identified miRNAs / Chen Wang and Jinggui Fang
Probing small non-coding RNAs structures / Jean-Vincent Philippe [and three others]
cDNA library generation for the analysis of small RNAs by high-throughput sequencing / Jennifer Gebetsberger, Roger Fricker, and Norbert Polacek
CLIP-Seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells / Jérôme Saulière and Hervé Le Hir
Microarray analysis of small non-coding RNAs / Michael Karbiener and Marcel Scheideler
RML-RACE, PPM-RACE, and qRT-PCR : an integrated strategy to accurately validate miRNA target genes / Chen Wang and Jinggui Fang
Dual luciferase gene reporter assays to study miRNA function / Thomas Clément, Véronique Salone, and Mathieu Rederstorff
Gene expression knockdown by transfection of siRNAs into mammalian cells / Yoann Abel and Mathieu Rederstorff
Efficient and selective knockdown of small non-coding RNAs / Xue-Hai Liang, Wen Shen, and Stanley T. Crooke
Cell-SELEX : in vitro selection of synthetic small specific ligands / Helena Dickinson [and three others]
Small non-coding RNAs and aptamers in diagnostics and therapeutics / Marissa Leonard, Yijuan Zhang, and Xiaoting Zhang.