Bookedited by Anton Posch.
Contents:
Mechanical/physical methods of cell distribution and tissue homogenization / Stanley Goldberg
Sample preservation through heat stabilization of proteins : principles and examples / Mats Borén
Isolating peripheral lymphocytes by density gradient centrifugation and magnetic cell sorting / Frederic Brosseron, Katrin Marcus, and Caroline May
Investigating the adipose tissue secretome : a protocol to generate high-quality samples appropriate for comprehensive proteomic profiling / Simon Göddeke, Jorg Kotzka, and Stefan Lehr
Methods for proteomics-based analysis of the human muscle secretome using an in vitro exercise model / Mika Scheler ... [et al.]
Urinary pellet sample preparation for shotgun proteomic analysis of microbial infection and host-pathogen interactions / Yanbao Yu and Rembert Pieper
Protocol for the parallel isolation of intact mitochondria from rat liver, kidney, heart, and brain / Sabine Schulz ... [et al.]
Isolation of mitochondria from cultured cells and liver tissue biopsies for molecular and biochemical analyses / Sabine Schmitt ... [et al.]
Dynamic range compression with ProteoMiner : principles and examples / Lei Li
Qualitative and quantitative proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue / Omid Azimzadeh, Michael J. Atkinson, and Soile Tapio
Full-length protein extraction protocols and gel-based downstream applications in formalin-fixed tissue proteomics / Alessandro Tanca, Sergio Uzzau, and Maria Filippa Addis
Enrichment of low-abundant protein targets by immunoprecipitation upstream of mass spectrometry / Barbara Kaboord ... [et al.]
Principles of protein labeling techniques / Christian Obermaier, Anja Griebel, and Reiner Westermeier
Isolation of extracellular vesicles for proteomic profiling / Dong-Sic Choi and Yong Song Gho
Protocol for exosome isolation and characterization : evaluation of ultracentrifugation, density-gradient separation, and immunoaffinity capture methods / David W. Greening ... [et al.]
Chloroplast isolation and affinity chromatography for enrichment of low-abundant proteins in complex proteomes / Roman G. Bayer, Simon Stael, and Markus Teige
Depletion of RuBisCO protein using the protamine sulfate precipitation method / Ravi Gupta and Sun Tae Kim
Step-by-step preparation of proteins for mass spectrometric analysis / Thomas Franz and Xinping Li
Identification of protein N-Termini using TMPP or dimethyl labeling and mass spectrometry / Jingjing Deng ... [et al.]
Optimization of cell lysis and protein digestion protocols for protein analysis by LC-MS/MS / Dominic Winter, Alireza Dehghani, and Hanno Steen
Comprehensive protocol to simultaneously study protein phosphorylation, acetylation, and n-linked sialylated glycosylation / Marcella Nunes Melo-Braga ... [et al.]
Protein profiling and phosphoprotein analysis by isoelectric focusing / Giuseppina Maccarrone and Michaela D. Filiou
Principles and examples of gel-based approaches for phosphoprotein analysis / Birgit Steinberger and Corina Mayrhofer
Neutral phosphate-affinity SDS-PAGE system for profiling of protein phosphorylation / Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
Enrichment and identification of bacterial glycopeptides by mass spectrometry / Nichollas E. Scott and Stuart J. Cordwell
In-gel peptide IEF sample preparation for LC/MS analysis / Tom Berkelman, Sricharan Bandhakavi, and Aran Paulus
Western blotting using in-gel protein labeling as a normalization control : stain-free technology / Jennifer E. Gilda and Aldrin V. Gomes
2-D western blotting for evaluation of antibodies developed for detection of host cell protein / Tom Berkelman, Adriana Harbers, and Sricharan Bandhakavi
Free llow electrophoresis for separation of native membrane protein complexes / Lutz Andreas Eichacker ... [et al.]
Three-dimensional electrophoresis for quantitative profiling of complex proteomes / Sergio Mauro ... [et al.]
Bead-based multiplex sandwich immunoassay to assess the abundance and posttranslational modification state of [beta]-catenin / Nicola Groll ... [et al.]
Identification of SUMO E3 ligase-specific substrates using the huprot human proteome microarray / Eric Cox ... [et al.]
Amyloid-binding proteins : affinity-based separation, proteomic identification, and optical biosensor validation / Alexei Medvedev ... [et al.]
Proteomic profiling by nanomaterials-based matrix- assisted laser desorption/ionization mass spectrometry for high-resolution data and novel protein information directly from biological samples / Suresh Kumar Kailasa and Hui-Fen Wu.