Bookedited by Gabriela K. Popescu.
Contents:
1. Assaying AMPA Receptor Oligomerization / Catherine L. Salussolia, Quan Gan, Lonnie P. Wollmuth
2. A Step-by-Step Guide to Single-Subunit Counting of Membrane-Bound Proteins in Mammalian Cells / Mark R. Aurousseau, Hugo McGuire, Rikard Blunck, Derek Bowie
3. Counting NMDA Receptors at the Cell Surface / Martin Horak, Young Ho Suh
4. Electrophysiological Tagging of Ionotropic Glutamate Receptors / Andres Barria
5. Electron Microscopy Analysis of AMPA Receptors in Dendritic Spines / Audra A. Kramer, Amber N. Petersen, Nashaat Z. Gerges
6. Functional Detection of Novel Triheteromeric NMDA Receptors / Sanjay S. Kumar
7. Expression, Purification, and Crystallization of Full Length Ionotropic Glutamate Receptors / Maria V. Yelshanskaya, Kei Saotome, Minfen Li, Alexander I. Sobolevsky
8. NMR Approaches to Functional Dynamics of Genetically Separated iGluR Domains / Christopher P. Ptak, Ahmed H. Ahmed, Robert E. Oswald
9. Computing Conformational Free Energies of iGluR Ligand-Binding Domains / Alvin Yu, Tyler Wied, John Belcher, Albert Y. Lau
10. LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors / Rita E. Sirrieh, Vasanthi Jayaraman
11. Assaying the Energetics of NMDA Receptor Pore Opening / Rashek Kazi, Melissa Daniel, Lonnie P. Wollmuth
12. Constructing a Rapid Solution Exchange System / David M. MacLean
13. Assessing The Effects of Ligand-Binding Mutations to AMPA and Kainate Receptor Kinetics / Mark W. Fleck
14. Analysis of Whole-Cell NMDA Receptor Currents / Vojtech Vyklicky, Miloslav Korinek, Ales Balik, Tereza Smejkalova, Barbora Krausova, Ladislav Vyklicky
15. Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses / Kelly A. Krogh, Stanley A. Thayer
16. Timing AMPA Receptor Activation with Laser-Pulse Photolysis / Li Niu
17. Current Recording and Kinetic Analyses for Single AMPA Receptors / Kinning Poon, Robert E. Oswald, Linda M. Nowak
18. Extracting Rate Constants for NMDA Receptor Gating from One-Channel Current Recordings / Kirstie A. Cummings, Gary J. Iacobucci, Gabriela K. Popescu.