Bookedited by Graeme L. Conn.
Contents:
Purification of RNA expressed in vivo inserted in a tRNA scaffold / Luc Ponchon and Frederic Dardel
Selective RNase H cleavage of target RNAs from a tRNA scaffold / Luc Ponchon [and others]
Preparation of long templates for RNA in vitro transcription by recursive PCR / Jessica C. Bowman [and others]
General protocols for preparation of plasmid DNA template, RNA in vitro transcription, and RNA purification by denaturing PAGE / Jo L. Linpinsel and Graeme L. Conn
Preparation of short RNA by in vitro transcription / Cheng Lu and Pingwei Li
Native RNA purification by gel filtration chromatography / Evan P. Booy, Hui Meng, and Sean A. McKenna
Cis-acting ribozymes for the production of RNA in vitro transcripts with defined 5ʹ and 3ʹ ends / Johanna M. Avis, Graeme L. Conn, and Scott C. Walker
Trans-acting antigenomic HDV ribozyme for production of in vitro transcripts with homogenous 3ʹ ends / Milena Szafraniec [and others]
Rapid preparation of RNA samples using DNA-affinity chromatography and DNAzyme methods / Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong
Preparation of lN-GST fusion protein for affinity immobilization of RNA / Genevieve Di Tomasso [and others]
Affinity purification of RNA using an ARiBo tag / Genevieve Di Tomasso [and others]
Plasmid template design and in vitro transcription of short RNAs within a "structure cassette" for structure probing experiments / Virginia K. Vachon and Graeme L. Conn
In vitro transcription of modified RNAs / Stephanie L. Moon and Jeffrey Wilusz
End-labeling oligonucleotides with chemical tags after synthesis / N. Ruth Zearfoss and Sean P. Ryder
High-purity enzymatic synthesis of site-specifically modified tRNA / Ya-Ming Hou
Se-derivatized RNAs for x-ray crystallography / Lina Lin and Zhen Huang
Biosynthetic preparation of 13C/15N-labeled rNTPs for high-resolution NMR studies of RNAs / Luigi Martino and Maria R. Conte
Preparative separation of ribonucleoside monophosphates by ion-pair reverse-phase HPLC / Pierre Dagenais and Pascale Legault
Splint ligation of RNA with T4 DNA ligase / Christopher J. Kershaw and Raymond T. O'Keefe.