Bookedited by Matt Dickinson, Jennifer Hodgetts.
Contents:
The phytoplasmas: an introduction / Matt Dickinson, Melanie Tuffen, and Jennifer Hodgetts
Techniques for the maintenance and propagation of phytoplasmas in glasshouse collections of Catharanthus roseus / Jennifer Hodgetts, David Crossley, and Matt Dickinson
Micropropagation and maintenance of phytoplasmas in tissue culture / Assunta Bertaccini, Samanta Paltrinieri, Marta Martini, Mara Tedeschi, and Nicoletta Contaldo
Dodder transmission of phytoplasmas / Jaroslava Přibylová and Josef Špak
Insect maintenance and transmission / Heather Kingdom
Capturing insect vectors of phytoplasmas / Phyllis Weintraub and Jürgen Gross
Insect vector transmission assays / Domenico Bosco and Rosemarie Tedeschi
Molecular identification of phytoplasma vector species / Sabrina Bertin and Domenico Bosco
Dienes' staining and light microscopy for phytoplasma visualization / Rita Musetti
DAPI staining and fluorescence microscopy techniques for phytoplasmas / Nancy M. Andrade and Nolberto L. Arismendi
Visualization of phytoplasmas using electron microscopy / B. Jean Devonshire
Automated DNA extraction for large numbers of plant samples / Nataša Mehle, Petra Nikolić, Matevž Rupar, Jana Boben, Maja Ravnikar, and Marina Dermastia
DNA extraction from arborescent monocots and how to deal with other challenging hosts / Nigel A. Harrison, Robert E. Davis, and Ericka E. Helmick
Nested PCR and RFLP analysis based on the 16S rRNA gene / Bojan Duduk, Samanta Paltrinieri, Ing-Ming Lee, and Assunta Bertaccini
PCR and RFLP analyses based on the ribosomal protein operon / Marta Martini and Ing-Ming Lee
Tuf and secY PCR amplification and genotyping of phytoplasmas / Xavier Foissac, Jean-Luc Danet, Sylvie Malembic-Maher, Pascal Salar, Dana Šafářová, Pavla Válová, and Milan Navrátil
PCR analysis of phytoplasmas based on the secA gene / Matt Dickinson and Jennifer Hodgetts
Single-strand conformation polymorphism analysis for differentiating phytoplasma strains / Martina Šeruga Musić and Dijana Škorić
Microarrays for universal detection and identification of phytoplasmas / Mogens Nicolaisen, Henriette Nyskjold, and Assunta Bertaccini
T-RFLP for detection and identification of phytoplasmas in plants / Jennifer Hodgetts and Matt Dickinson
Real-time PCR for universal phytoplasma detection and quantification / Nynne Meyn Christensen, Henriette Nyskjold, and Mogens Nicolaisen
A real-time PCR detection system for the bois noir and flavescence dorée phytoplasmas and quantification of the target DNA / Nataša Mehle, Nina Prezelj, Matjaž Hren, Jana Boben, Kristina Gruden, Maja Ravnikar, and Marina Dermastia
Real-time PCR for specific detection of three phytoplasmas from the apple proliferation group / Nataša Mehle, Petra Nikolić, Kristina Gruden, Maja Ravnikar, and Marina Dermastia
Reverse transcription-PCR for phytoplasma detection utilizing crude sap extractions / Paolo Margaria and Sabrina Palmano
In-field diagnostics using loop-mediated isothermal amplification / Jenny Tomlinson
DNA bar-coding for phytoplasma identification / Olga Makarova, Nicoletta Contaldo, Samanta Paltrinieri, Assunta Bertaccini, Henriette Nyskjold, and Mogens Nicolaisen
Phylogenetic analyses of phytoplasmas based on whole-genome comparison / Hiromi Nishida
The i PhyClassifier, an interactive online tool for phytoplasma classification and taxonomic assignment / Yan Zhao, Wei Wei, Ing-Ming Lee, Jonathan Shao, Xiaobing Suo, and Robert E. Davis
Phytoplasma proteomic analysis / Xianling Ji and Yingping Gai
Preparation of phytoplasma membrane recombinant proteins / Luciana Galetto, Majid Siampour, and Cristina Marzachì
Phytoplasma plasmid DNA extraction / Mark T. Andersen and Lia W. Liefting
Cesium chloride-bisbenzimide gradients for separation of phytoplasma and plant DNA / Lucy T.T. Tran-Nguyen and Bernd Schneider
Pulsed-field gel electrophoresis for isolation of full-length phytoplasma chromosomes from plants / Carmine Marcone
Mapping the phytoplasma chromosome / Sylvie Malembic-Maher and Patricia Carle.