Bookedited by Anne Straube.
Contents:
How to measure microtubule dynamics? / Anne Straube
Purification of tubulin from porcine brain / Christopher Gell [and others]
Purification of tubulin from the fission yeast Schizosaccharomyces pombe / Douglas R. Drummond [and others]
Generation of differentially polyglutamylated microtubules / Benjamin Lacroix and Carsten Janke
Preparation of pure tyrosinated or detyrosinated tubulin isoforms / Laurence Lafanechère and Didier Job
[alpha][beta]-tubulin and microtubule-binding assays / Jaime N. Campbell and Kevin C. Slep
Analysis of microtubule assembly kinetics using turbidimetry / Felicia Gaskin
Isolation of centrosomes from cultured cells / Simone Reber
Preparation of dual-color polarity-marked fluorescent microtubule seeds / Miho Katsuki, Etsuko Muto, and Robert A. Cross
Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy / Ivo A. Telley, Peter Bieling, and Thomas Surrey
Force generation by dynamic microtubules in vitro / Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom
Variations on the single-molecule assay for microtubule-associated proteins and kinesins / Susanne Bechstedt [and others]
Analysing the ATP turnover cycle of microtubule motors / Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard
Cryo-electron tomography of microtubules assembled in vitro from purified components / Frédéric M. Coquelle [and others]
Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy / Andreas Merdes and Jan De Mey
Photoactivatable-GFP-[alpha]-tubulin as a tool to study microtubule plus-end turnover in living human cells / Catarina P. Samora and Andrew D. McAinsh
Quantification of asymmetric microtubule nucleation at subcellular structures / Xiaodong Zhu and Irina Kaverina
Investigating microtubule dynamic instability using microtubule-targeting agents / Stéphane Honore and Diane Braguer
Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system / Nicola Maghelli and Iva M. Tolić-Nørrelykke
Isolation, identification, and validation of microtubule-associated proteins from Drosophila embryos / Robin Antrobus and James G. Wakefield
Isolation of novel +TIPs and their binding partners using affinity purification techniques / Ka Lou Yu [and others].