Bookedited by Tanya Parish and David M. Roberts.
Contents:
Whole-genome sequencing for comparative genomics and de novo genome assembly / Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn
Whole-transcriptome sequencing for high-resolution transcriptomic analysis in mycobacterium tuberculosis / Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn
RNA sequencing for transcript 5'-end mapping in mycobacteria / Scarlet S. Shell [and 3 otherst]
Fractionation and analysis of mycobacterial proteins / Megan C. Lucas [and 6 others]
Lipid and lipoarabinomannan isolation and characterization / Marie-Antoinette Lanéelle, Jérôme Nigou, and Mamadou Daffé
Metabolomics of mycobacterium tuberculosis / Madhumitha Nandakumar [and 3 others]
Electroporation of mycobacteria / Renan Goude, David M. Roberts, and Tanya Parish
Targeted gene knockout and essentiality testing by homologous recombination / Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi
Construction of conditional knockdown mutants in mycobacteria / Dirk Schnappinger, Kathryn M. O'Brien, and Sabine Ehrt
Mycobacterial recombineering / Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti
In vitro models that utilize hypoxia to induce non-replicating persistence in mycobacteria / Charles D. Sohaskeyand Martin I. Voskuil
Genetic dissection of mycobacterial biofilms / Anil K. Ojha, William R. Jacobs Jr., and Graham F. Hatfull
Measuring efflux and permeability in mycobacteria / Liliana Rodrigues, Miguel Viveiros, and José A. Aínsa
Single-cell analysis of mycobacteria using microfluidics and time-lapse microscopy / Neeraj Dharand and Giulia Manina
Antimicrobial susceptibility testing for Mycobacterium sp. / Delia Blanco-Ruano [and 6 others]
Determination of compound kill kinetics against mycobacterium tuberculosis / Julie Early and Torey Alling
Microplate alamar blue assay (MABA) and low oxygen recovery assay (LORA) for mycobacterium tuberculosis Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau
Multi-stress model for high throughput screening against non-replicating mycobacterium tuberculosis / Ben Gold, Thulasi Warrier, and Carl Nathan
Isolation and characterization of compound-resistant isolates of mycobacterium tuberculosis / Theresa O'Malley and Eduard Melief
Macrophage infection models for mycobacterium tuberculosis / Benjamin K. Johnson and Robert B. Abramovitch
Infection of human neutrophils to study virulence properties of mycobacterium tuberculosis / Tobias Dallenga, Björn Corleis, and Ulrich E. Schaible
Isolation of bead phagosomes to study virulence function of M. tuberculosis cell wall lipids / Anna C. Geffken, Emmanuel C. Patin, and Ulrich E. Schaible
Live imaging of mycobacterium marinum infection in dictyostelium discoideum / Caroline Barisch, Ana T. López-Jiménez, and Thierry Soldati
Testing chemical and genetic modulators in mycobacterium tuberculosis infected cells using phenotypic assays / Vincent Delorme [and 3 others].