Bookedited by Helena Jane Maier, Erica Bickerton, Paul Britton.
Contents:
Coronaviruses : an overview of their replication and pathogenesis / Anthony R. Fehr and Stanley Perlman
Identification of a novel coronavirus from Guinea Fowl using metagenomics / Mariette F. Ducatez and Jean-Luc Guérin
Serological diagnosis of feline coronavirus infection by immunochromatographic test / Tomomi Takano and Tsutomu Hohdatsu
Estimation of evolutionary dynamics and selection pressure in coronaviruses / Muhammad Munir and Martí Cortey
Preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration / Ruth M. Hennion
Preparation of chicken kidney cell cultures for virus propagation / Ruth M. Hennion and Gillian Hill
Isolation and propagation of coronaviruses in embryonated eggs / James S. Guy
Characterization of human coronaviruses on well- differentiated human airway epithelial cell cultures / Hulda R. Jonsdottir and Ronald Dijkman
Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo / Joeri Kint, Helena Jane Maier, and Erik Jagt
Purification of coronavirus virions for Cryo-EM and proteomic analysis / Stuart Dent and Benjamin W. Neuman
Partial purification of IBV and subsequent isolation of viral RNA for next-generation sequencing / Sarah M. Keep, Erica Bickerton, and Paul Britton
Transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses / Sarah M. Keep, Erica Bickerton, and Paul Britton
Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes / Fernando Almazán ... [et al.]
Protein histochemistry using coronaviral spike proteins : studying binding profiles and sialic acid requirements for attachment to tissues / Iresha N. Ambepitiya Wickramasinghe and M. Hélène Verheije
Identification of protein receptors for coronaviruses by mass spectrometry / V. Stalin Raj ... [et al.]
Single particle tracking assay to study coronavirus membrane fusion / Deirdre A. Costello and Susan Daniel
Studying coronavirus-host protein interactions / Chee-Hing Yang ... [et al.]
Field-proven yeast two-hybrid protocol used to identify coronavirus-host protein-protein interactions / Pierre- Olivier Vidalain ... [et al.]
Investigation of the functional roles of host cell proteins involved in coronavirus infection using highly specific and scalable RNA interference (RNAi) approach / Jean Kaoru Millet and Béatrice Nal
Transcriptome analysis of feline infectious peritonitis virus infection / Parvaneh Mehrbod ... [et al.]
Quantification of interferon signaling in avian cells / Joeri Kint and Maria Forlenza
Studying the dynamics of coronavirus replicative structures / Marne C. Hagemeijer and Cornelis A. M. de Haan
Preparation of cultured cells using high-pressure freezing and freeze substitution for subsequent 2D or 3D visualization in the transmission electron microscope / Philippa C. Hawes.