Bookedited by Mathieu Vinken, Vera Rogiers.
Contents:
Isolation and culture of mouse hepatocytes
Cryopreservation of hepatocytes
Culture conditions promoting proliferation and cell cycle synchronization
Immortalized human hepatic cell lines for In vitro testing and research purposes
Culture and functional characterization of human hepatoma hepG2s
Establishment and characterization of an in vitro model of Fas-mediated hepatocyte cell death
Serum-free directed differentiation of human embryonic stem cells to hepatocytes
Human skin-derived precursor Cells: isolation, expansion, and hepatic differentiation
Generation of hepatocytes from pluripotent stem cells for drug screening and developmental modeling
Differentiation-promoting medium additives for hepatocyte
Cultivation and cryopreservation
Coculture and long-term maintenance of hepatocytes
Primary hepatocytes in sandwich culture
Establishing liver bioreactors for In Vitro Research
Epigenetic modifications as antidedifferentiation strategy for primary hepatocytes in culture
Transfection of Primary Hepatocytes with Liver-enriched transcription factors using adenoviral vectors
Transcriptomics of hepatocytes treated with Toxicants for Investigating Molecular mechanisms underlying hepatotoxicity
Global microRNA analysis in primary hepatocyte Cultures
Mass Spectrometry-Based proteomics for telative protein quantification and biomarker Identification in primary human Hepatocytes
Targeted metabolomics for homocysteine-related metabolites in primary hepatocytes
Measurement of cytochrome P450 Enzyme Induction and Inhibition in human hepatoma cells
Analysis of sinusoidal drug uptake transporter activities in Primary Human Hepatocytes
Measurement of albumin secretion as functionality test in primary hepatocyte cultures
Measurembent of Blood Coagulation Factor Synthesis in cultures of human hepatocytes
Functionality testing of primary hepatocytes in Culture by Measuring Urea Synthesis
Assay of Bile acid conjugation and excretion in human hepatocytes
General cytotoxicity assessment by Means of the MTT assay
Measurement of apoptotic and necrotic cell death in primary hepatocyte cultures
Critical factors in the Assessment of cholestatic liver injury In vitro
In vitro cell culture models of hepatic steatosis
Assessment of liver fibrotic Insults In Vitro.