Bookedited by Manfred Heinlein.
Contents:
Plasmodesmata : channels for intercellular signaling during plant growth and development / Iris Sevilem, Shri Ram Yadav, and Ykä Helariutta
Plasmodesmata : channels for viruses on the move / Manfred Heinlein
Imaging plasmodesmata with high-resolution scanning electron microscopy / Deborah A. Barton and Robyn L. Overall
Preparative methods for imaging plasmodesmata at super-resolution / Karen Bell and Karl Oparka
Isolation of plasmodesmata from Arabidopsis suspension culture cells / Magali S. Grison, Lourdes Fernandez-Calvino, Sébastien Mongrand, and Emmanuelle M.F. Bayer
Immunofluorescence detection of callose deposition around plasmodesmata sites / Ali Pendle and Yoselin Benitez-Alfonso
Imaging callose at plasmodesmata using aniline blue : quantitative confocal microscopy / Raul Zavaliev and Bernard L. Epel
Localization of fluorescently tagged protein to plasmodesmata by correlative light and electron microscopy / Shannon Modla, Jeffrey L. Caplan, Kirk J. Czymmek, and Jung-Youn Lee
Quantification of plant cell coupling with live-cell microscopy / Johannes Liesche and Alexander Schulz
Drop-ANd-See : a simple, real-time, and noninvasive technique for assaying plasmodesmal permeability / Weier Cui, Xu Wang, and Jung-Youn Lee
Mapping symplasmic fields at the shoot apical meristem using iontophoresis and membrane potential measurements / Christiaan van der Schoot and Päivi L. H. Rinne
Analysis of the conductivity of plasmodesmata by microinjection / Friedrich Kragler
Investigating plasmodesmata genetics with virus-induced gene silencing and an agrobacterium-mediated GFP movement assay / Jacob O. Brunkard, Tessa M. Burch-Smith, Anne M. Runkel, and Patricia Zambryski
Probing plasmodesmata function with biochemical inhibitors / Rosemary G. White
GAL4 transactivation-based assay for the detection of selective intercellular protein Movement / Dhinesh Kumar, Huan Chen, Yeonggil Rim, and Jae-Yean Kim
Techniques for assessing the effects of pharmacological inhibitors on intercellular protein movement / Shuang Wu and Kimberly L. Gallagher
Probing protein targeting to plasmodesmata using fluorescence recovery after photo-bleaching / Kathryn M. Wright and Katrin M. MacKenzie
Tracking of intercellular small RNA movement / Christophe Himber and Patrice Dunoyer
Analysis of the role of myosins in targeting proteins to plasmodesmata / Martin Di Donato and Khalid Amari
Pumilio-based RNA in vivo imaging / Jens Tilsner
In vivo RNA labeling using MS2 / Eduardo Peña, Manfred Heinlein, and Adrian Sambade.