Bookedited by Boye Schnack Nielsen, Bioneer A/S, Molecular Histology, Hørholm, Denmark.
Summary: "In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions."--Page [4] of cover.
Contents:
Fixation/permeabilization procedure for mRNA in situ hybridization of zebrafish whole-mount oocytes, embryos, and larvae / Ricardo Fuentes and Juan Fernández
MicroRNA in situ hybridization on whole-mount preimplantation embryos / Karen Goossens, Luc Peelman, and Ann Van Soom
Whole-mount in situ hybridization (WISH) optimized for gene expression analysis in mouse embryos and embryoid bodies / Eleni Dakou [and five others]
Whole-mount in situ hybridization using DIG-labeled probes in planarian / Agnieszka Rybak-Wolf and Jordi Solana
In situ hybridization on whole-mount zebrafish embryos and young larvae / Bernard Thisse and Christine Thisse
LNA-based in situ hybridization detection of mRNAs in embryos / Diana K. Darnell and Parker B. Antin
Chromogen detection of microRNA in frozen clinical tissue samples using LNA probe technology / Boye Schnack Nielsen, Trine Møller, and Kim Holmstrøm
MicroRNA in situ hybridization in tissue microarrays / Julia J. Turnock-Jones and John P.C. Le Quesne
Fluorescence in situ hybridization for detection of small RNAs on frozen tissue sections / Asli Silahtaroglu
Sensitive and specific in situ hybridization for early drug discovery / Pernille A. Usher ... [and six others]
Zinc-based fixation for high-sensitivity in situ hybridization : a nonradioactive colorimetric method for the detection of rare transcripts on tissue sections / Electra Stylianopoulou, George Skavdis, and Maria Grigoriou
Dual-color ultrasensitive bright-field RNA in situ hybridization with RNAscope / Hongwei Wang ... [and eight others]
Fully automated fluorescence-based four-color multiplex assay for co-detection of microRNA and protein biomarkers in clinical tissue specimens / Lorenzo F. Sempere
Multiplexed miRNA fluorescence in situ hybridization for formalin-fixed paraffin-embedded tissues / Neil Renwick ... [and three others]
Simultaneous detection of nuclear and cytoplasmic RNA variants utilizing stellaris RNA fluorescence in situ hybridization in adherent cells / Sally R. Coassin ... [and three others]
Quantitative ultrasensitive bright-field RNA in situ hybridization with RNAscope / Hongwei Wang ... [and eight others]
Identification of low-expressing transcripts of the NPY receptors' family in the murine lingual epithelia / Sergei Zolotukhin
In situ hybridization for fungal ribosomal RNA sequences in paraffin-embedded tissues using biotin-labeled locked nucleic acid probes / Kathleen T. Montone
In situ hybridization freeze-assisted punches (IFAP) : technique for liquid-based tissue extraction from thin slide-mounted sections for DNA methylation analysis / Lawrence S. Own and Paresh D. Patel
miRNA detection at single-cell resolution using microfluidic LNA Flow-FISH / Meiye Wu, Matthew E. Piccini, and Anup K. Singh
PNA-based fluorescence in situ hybridization for identification of bacteria in clinical samples / Mustafa Fazli ... [and four others].