Bookedited by Gordon G. Carmichael.
Contents:
Diverse functions and mechanisms of mammalian long noncoding RNAs
Sequence-defined oligoaminoamides for the delivery of siRNAs
A biochemical approach to identify direct microRNA targets
Native gel analysis for mammalian microRNPs assembled from pre-microRNAs
Identification and validation of miRNA target sites within nontraditional miRNA targets
Fractionation of non-polyadenylated and ribosomal-free RNAs from mammalian cells
Affinity purification of long noncoding RNA-protein complexes from formaldehyde cross-linked mammalian cells
RNA pulldown protocol for in vitro detection and identification of RNA-associated proteins
nRIP-seq: A technique to identify RNA targets of an RNA binding protein on a genome-wide scale
Analysis of the subcellular distribution of RNA by fluorescence in situ hybridization
RNA fluorescence in situ hybridization in cultured mammalian cells
Locating RNAs in situ with FISH-STIC probes
Rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells with antisense oligonucleotides
On using magnesium and potassium ions in RNA experiments
Studying the affinity, kinetic stability, and specificity of RNA/protein interactions: SINE ncRNA/Pol II complexes as a model system