Bookedited by Elena Ranieri.
Contents:
Negative and positive separation techniques for the isolation of antigen-specific CD8+ T cells from blood and tumor tissue / Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri
In vitro\ex vivo generation of cytotoxic T lymphocytes / Margherita Gigante and Elena Ranieri
Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry / Iulia Popescu [and three others]
Intracellular signaling of CTLs / Sharon Natasha Cox
Monitoring antigen-specific T cell responses using real-time PCR / Devin B. Lowe, Jennifer L. Taylor, and Walter J. Storkus
CTL ELISPOT assay / Elena Ranieri, Iulia Popescu, and Margherita Gigante
Methods of purification of CTL-derived exosomes / Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli
Characterization of CTL by microscopy / Giuseppe Stefano Netti
Intravital imaging of cytotoxic T lymphocytes / Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi
The microarray-based approach for the analysis of the transcriptome / Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo
miRNome analysis using real-time PCR / Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo
Proteomic approaches by SELDI and MALDI-TOF/MS for CTL analysis / Massimo Papale and Maria Teresa Rocchetti
Two-dimensional gel electrophoresis approach for CTL phosphoproteome analysis / Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo
Targeting the JAK/STAT pathway in cytotoxic T lymphocytes (CTL) by next generation sequencing (NGS) / Maddalena Gigante, Sterpeta Diella, and Elena Ranieri
Experimental model for studying the involvement of regulatory cytotoxic T cells in bone resorption / Giacomina Brunetti [and three others]
CTL and transplantation : tissue in vivo characterization / Federica Rascio, Chiara Divella, and Giuseppe Grandaliano.