Bookedited by M. Lucrecia Alvarez, Mahtab Nourbakhsh.
Contents:
Part I Structural RNA mapping
Full-length characterization of transcribed genomic region
Rapid mapping of RNA 3 and 5 ends
Single nucleotide mapping of RNA 5 and 3 ends
Analysis of RNA secondary structure
Tertiary structure mapping of the Pri-miRNA miR-17-92
In situ hybridization detection of miRNA using LNA [trademark] oligonucleotides
Quantification of miRNAs by a simple and specific qPCR method
RNA isolation for small RNA next-generation sequencing from acellular biofluids
Sequencing small RNA : introduction and data analysis fundamentals
Measuring expression levels of small regulatory RNA molecules from body fluids and formalin-fixed, paraffin-embedded samples
MicroRNA profiling in plasma or serum using quantitative RT-PCR
MicroRNA profiling of exosomes isolated from biofluids and conditioned media
Isolation of urinary exosomes for RNA biomarker discovery using a simple, fast, and highly scalable method
Part II Functional RNA mapping
Identification of actively translated mRNAs
Mapping of internal ribosome entry sites (IRES)
Mapping of protein binding RNA elements
Purification of RNA-binding proteins
De novo approach to classify protein-coding and noncoding transcripts based on sequence composition
Computational methods to predict long noncoding RNA functions based on co-expression network
MicroRNA biogenesis : dicing assay
Faster experimental validation of microRNA targets using cold fusion cloning and a dual firefly-Renilla luciferase reporter assay
Experimental validation of predicted mammalian microRNAs of mirtron origin
A guide for miRNA target prediction and analysis using web-based applications
Tapping microRNA regulation networks through integrated analysis of microRNA-mRNA high-throughput profiles
miRWalk database for miRNA-target interactions
A schematic workflow for collecting information about the interaction between copy number variants and microRNAs using existing resources
SYBR green and TaqMan quantitative PCR arrays : expression profile of genes relevant to a pathway or a disease state
Comprehensive meta-analysis of microRNA expression using a robust rank aggregation approach.