Bookedited by Andrei I. Ivanov.
Contents:
Pharmacological inhibitors of exocytosis and endocytosis: novel bullets for old targets
Systematic analysis of endocytosis by cellular perturbations
Real-time detection of SNARE complex assembly with FRET using the tetracysteine system
Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides
Secretion of circular proteins using sortase
Fractionation of subcellular membrane vesicles of epithelial and non-epithelial cells by OptiPrep density gradient ultracentrifugation
Combining pulsed SILAC labeling and click-chemistry for quantitative secretome analysis
Probabilistic density maps to study the spatial organization of endocytosis
Use of Kaede and Kikume green-red fusions for live cell imaging of g protein-coupled receptors
HaloTag as a tool to investigate peroxisome dynamics in cultured mammalian cells
SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells
FlAsh-palm: Super-resolution pointillist imaging with FlAsh-tetracysteine labeling
Analysis of protein dynamics with tandem fluorescent protein timers
Synchronization of secretory cargos trafficking in populations of cells
Use of transmembrane fret to investigate the internalization of glycosylated proteins
A method to rapidly induce organelle-specific molecular activities and membrane tethering
A novel pair of split Venus fragments to detect protein-protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays
Real-time investigation of plasma membrane deformation and fusion pore expansion using polarized total internal reflection fluorescence microscopy
Nanocones to study initial steps of endocytosis
A novel permeabilization protocol to obtain intracellular 3d immunolabeling for electron tomography
VIS2FIX: Rapid chemical fixation of vitreous sections for immuno-electron microscopy
Chemical genomics: Characterizing target pathways for bioactive compounds using the endomembrane trafficking network
Application of RNAi technology and fluorescent protein markers to study membrane traffic in c. Elegans
Visualization of clathrin-mediated endocytosis in live drosophila egg chambers
A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling
Microfluidic devices for imaging trafficking events in vivo using genetic model organisms
The "in situ" proximity ligation assay to probe protein-protein interactions in intact tissues
Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy
Measurement of dynamic F-actin changes during exocytosis.