Bookedited by Alain Filloux, Juan-Luis Ramos.
Contents:
Gene transfer : transduction / Emanuela Frangipani
Gene transfer : transformation/electroporation / Frédéric Cadoret, Chantal Soscia, and Romé Voulhoux
Gene transfer : conjugation / Thibault G. Sana, Aurélie Laubier, and Sophie Bleves
Pseudomonas bacteriophage isolation and production / Joana Azeredo, Sanna Sillankorva, and Diana P. Pires
Genotyping methods / Burkhard Tümmler
Drug susceptibility testing by dilution methods / Katy Jeannot and Patrick Plésiat
Plate-based assay for swimming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole
Plate-based assay for swarming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole
Motility assay : twitching motility / Lynne Turnbull and Cynthia B. Whitchurch
Qualitative and quantitative assays for flagellum- mediated chemotaxis / José Antonio Reyes Darias [and five others]
Microscopic analysis : morphotypes and cellular appendages / Olga Zaborina, John Alverdy, Megha Shah, and Yimei Chen
Determination of lipolytic enzyme activities / Karl-Erich Jaeger and Filip Kovacic
Elastinolytic and proteolytic enzymes / Efrat Kessler and Mary Safrin
In vitro assays to monitor the activity of Pseudomonas aeruginosa type III secreted proteins / Stephanie L. Rolsma and Dara W. Frank
Cell fractionation / Bérengère Ize, Véronique Viarre, and Romé Voulhoux
Characterization of molecular interactions using isothermal titration calorimetry / Tino Krell [and seven others]
Proteomic analysis / Pascal Cosette and Thierry Jouenne
Membrane proteomics of Pseudomonas aeruginosa / Zofia Magnowska, Isabel Hartmann, Lothar Jänsch, and Dieter Jahn
Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays / Sophie de Bentzmann and Christophe Bordi
Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules / Matthew Fletcher [and tree others]
LC-MS/MS quantitative analysis of quorum sensing signal molecules / Catharine A. Ortori [and four others]
LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP / Yasuhiko Irie and Matthew R. Parsek
Metabolic footprinting : extracellular metabolomic analysis / Volker Behrends, Huw D. Williams, and Jacob G. Bundy
Pyoverdine and pochelin measurements / Françoise Hoegy, Gaetan L. A. Mislin, and Isabelle J. Schalk
Measurement of phenazines in bacterial cultures / Suzanne E. Kern and Dianne K. Newman
Extraction and measurement of NAD(P)+ and NAD(P)H / Suzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman
Cyanide measurements in bacterial culture and sputum / Chandrika Goh Nair, Ben Ryall, and Huw D. Williams
Monitoring iron uptake by siderophores / Françoise Hoegy and Isabelle J. Schalk
Exopolysaccharide quantification / Irina Sadovskaya
Liquid chromatography/mass spectrometry for the identification and quantification of rhamnolipids / Ahmad Mohammad Abdel-Mawgoud, François Lépine, and Eric Déziel
LPS quantitation procedures / Joseph S. Lam, Erin M. Anderson, and Youai Hao
Monitoring lectin interactions with carbohydrates / Sophie de Bentzmann, Annabelle Varrot, and Anne Imberty
Mining the Pseudomonas genome / Geoffrey L. Winsor and Fiona S. L. Brinkman
Identification of bacterial small RNAs by RNA sequencing / María Gómez-Lozano [and three others.]
Gene amplification and qRT-PCR / Cerith Jones and Alain Filloux
The Standard European Vector Architecture (SEVA) plasmid toolkit / Gonzalo Durante-Rodríguez, Víctor de Lorenzo, and Esteban Martínez-García
Chromosomal integration of transcriptional fusions / Rafael Silva-Rocha and Víctor de Lorenzo
A method to capture large DNA fragments from genomic DNA / Geneviève Ball, Alain Filloux, and Romé Voulhoux
Transposon mutagenesis / Hemantha D. Kulasekara
Site-directed mutagenesis and gene deletion using reverse genetics / Daniela Muhl and Alain Filloux
Signature-tagged mutagenesis / Irena Kukavica-Ibrulj and Roger C. Levesque
Construction of a Pseudomonas aeruginosa genomic DNA library / Christophe Bordi
Strategy for genome sequencing analysis and assembly for comparative genomics of Pseudomonas genomes / Julie Jeukens [and three others]
Promoter fusions with optical outputs in individual cells and in populations / Ilaria Benedetti and Victor de Lorenzo
Chromatin immunoprecipitation for ChIP-chip and ChIP-seq / Sebastian Schulz and Susanne Häussler
Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans / Ashleigh MacKenzie [and three others]
Methods for studying biofilm formation : flow cells and confocal laser scanning microscopy / Tim Tolker-Nielsen and Claus Sternberg
Biofilm formation in the 96-well microtiter plate / Barbara M. Coffey and Gregory G. Anderson
Methods for studying biofilm dispersal in Pseudomonas aeruginosa / Nicolas Barraud [and three others]
Pseudomonas aeruginosa PA14 pathogenesis in Caenorhabditis elegans / Natalia V. Kirienko [and three others]
Assessing Pseudomonas aeruginosa virulence using a nonmammalian host : Dictyostelium discoideum / Geneviève Filion and Steve J. Charette
Assessing Pseudomonas virulence with nonmammalian host : Galleria mellonella / Gudrun Koch [and three others]
Assessing Pseudomonas virulence with the nonmammalian host model : Arabidopsis thaliana / Regina L. Baldini, Melissa Starkey, and Laurence G. Rahme
Assessing Pseudomonas aeruginosa persister/antibiotic tolerant cells / Ronen Hazan [and three others]
Assessing Pseudomonas virulence with nonmammalian host : Zebrafish / María A. Llamas and Astrid M. van der Sar
Assessing Pseudomonas virulence with a nonmammalian host : Drosophila melanogaster / Samantha Haller, Stefanie Limmer, and Dominique Ferrandon
Assessing Pseudomonas virulence using host cells / Iwona Bucior, Cindy Tran, and Joanne Engel
Assessing Pseudomonas aeruginosa virulence and the host response using murine models of acute and chronic lung infection / Irena Kukavica-Ibrulj [and four others]
Assessing Pseudomonas virulence using mammalian models : acute infection model / Antje Munder and Burkhard Tümmler
Burn mouse models / Henrik Calum, Niels Høiby, and Claus Moser.